Hypoxic/ischemic models in newborn piglet:: Comparison of constant FiO2 versus variable FiO2 delivery

被引:46
作者
Bjorkman, S. Tracey [1 ]
Foster, Kelley A. [1 ]
O'Driscoll, Stephanie M. [1 ]
Healy, Geneuieue N. [1 ]
Lingwood, Barbara E. [1 ]
Burke, Chris [1 ]
Colditz, Paul B. [1 ]
机构
[1] Univ Queensland, Royal Brisbane & Womens Hosp, Perinatal Res Ctr, Brisbane, Qld 4029, Australia
基金
英国医学研究理事会;
关键词
hypoxia/ischemia; newborn piglet;
D O I
10.1016/j.brainres.2006.04.119
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
A comparison of a constant (continuous delivery of 4% FiO(2)) and a variable (initial 5% FiO(2) with adjustments to induce low amplitude EEG (LAEEG) and hypotension) hypoxic/ischemic insult was performed to determine which insult was more effective in producing a consistent degree of survivable neuropathological damage in a newborn piglet model of perinatal asphyxia. We also examined which physiological responses contributed to this outcome. Thirty-nine 1-day-old piglets were subjected to either a constant hypoxic/ischemic insult of 30- to 37-min duration or a variable hypoxic/ischemic insult of 30-min low peak amplitude EEG (LAEEG < 5 mu V) including 10 min of low mean arterial blood pressure (MABP < 70% of baseline). Control animals (n = 6) received 21% FiO(2) for the duration of the experiment. At 72 h, the piglets were euthanased, their brains removed and fixed in 4% paraformaldehyde and assessed for hypoxic/ischemic injury by histological analysis. Based on neuropathology scores, piglets were grouped as undamaged or damaged; piglets that did not survive to 72 h were grouped separately as dead. The variable insult resulted in a greater number of piglets with neuropathological damage (undamaged = 12.5%, damaged = 68.75%, dead = 18.75%) while the constant insult resulted in a large proportion of undamaged piglets (undamaged = 50%, damaged = 22.2%, dead = 27.8%). A hypoxic insult varied to maintain peak amplitude EEG < 5 mu V results in a greater number of survivors with a consistent degree of neuropathological damage than a constant hypoxic insult. Physiological variables MABP, LAEEG, pH and arterial base excess were found to be significantly associated with neuropathological outcome. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:110 / 117
页数:8
相关论文
共 17 条
[1]  
DECOURTENMYERS GM, 1989, BIOMED BIOCHIM ACTA, V48, pS143
[2]   COMPARATIVE ASPECTS OF THE BRAIN GROWTH SPURT [J].
DOBBING, J ;
SANDS, J .
EARLY HUMAN DEVELOPMENT, 1979, 3 (01) :79-83
[3]   Influence of propofol on neuronal damage and apoptotic factors after incomplete cerebral ischemia and reperfusion in rats -: A long-term observation [J].
Engelhard, K ;
Werner, C ;
Eberspächer, E ;
Pape, M ;
Stegemann, U ;
Kellermann, K ;
Hollweck, R ;
Hutzler, P ;
Kochs, E .
ANESTHESIOLOGY, 2004, 101 (04) :912-917
[4]   An improved survival model of hypoxia/ischaemia in the piglet suitable for neuroprotection studies [J].
Foster, KA ;
Colditz, PB ;
Lingwood, BE ;
Burke, C ;
Dunster, KR ;
Roberts, MS .
BRAIN RESEARCH, 2001, 919 (01) :122-131
[5]   CEREBRAL HISTOLOGIC AND ELECTROCORTICOGRAPHIC CHANGES AFTER ASPHYXIA IN FETAL SHEEP [J].
GUNN, AJ ;
PARER, JT ;
MALLARD, EC ;
WILLIAMS, CE ;
GLUCKMAN, PD .
PEDIATRIC RESEARCH, 1992, 31 (05) :486-491
[6]  
Johnston MV, 1998, ASPHYXIA AND FETAL BRAIN DAMAGE, P113
[7]  
Laws PJ., 2004, AUSTR MOTHERS BABIES
[8]   Cerebral impedance and neurological outcome following a mild or severe hypoxic/ischemic episode in neonatal piglets [J].
Lingwood, BE ;
Dunster, KR ;
Healy, GN ;
Ward, LC ;
Colditz, PB .
BRAIN RESEARCH, 2003, 969 (1-2) :160-167
[9]   Determining the contribution of asphyxia to brain damage in the neonate [J].
Low, JA .
JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH, 2004, 30 (04) :276-286
[10]  
RAJU TNK, 1992, BIOL NEONATE, V62, P202, DOI 10.1159/000243873