Listeria monocytogenes σB Modulates PrfA-Mediated Virulence Factor Expression

被引:94
作者
Ollinger, Juliane [1 ]
Bowen, Barbara [1 ]
Wiedmann, Martin [1 ]
Boor, Kathryn J. [1 ]
Bergholz, Teresa M. [1 ]
机构
[1] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA
基金
美国国家卫生研究院;
关键词
TRANSCRIPTIONAL ACTIVATOR PRFA; TO-CELL SPREAD; STRESS CONDITIONS; GENE-EXPRESSION; IN-VITRO; DIFFERENTIAL REGULATION; BACTERIAL PATHOGENESIS; INTRACELLULAR GROWTH; ACID TOLERANCE; REGULATOR PRFA;
D O I
10.1128/IAI.01205-08
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Listeria monocytogenes sigma(B) and positive regulatory factor A (PrfA) are pleiotropic transcriptional regulators that coregulate a subset of virulence genes. A positive regulatory role for sigma(B) in prfA transcription has been well established; therefore, observations of increased virulence gene expression and hemolytic activity in a Delta sigB strain initially appeared paradoxical. To test the hypothesis that L. monocytogenes sigma(B) contributes to a regulatory network critical for appropriate repression as well as induction of virulence gene expression, genome-wide transcript profiling and follow-up quantitative reverse transcriptase PCR (qRT-PCR), reporter fusion, and phenotypic experiments were conducted using L. monocytogenes prfA*, prfA* Delta sigB, Delta prfA, and Delta prfA Delta sigB strains. Genome-wide transcript profiling and qRT-PCR showed that in the presence of active PrfA (PrfA*), sigma(B) is responsible for reduced expression of the PrfA regulon. sigma(B)-dependent modulation of PrfA regulon expression reduced the cytotoxic effects of a PrfA* strain in HepG2 cells, highlighting the functional importance of regulatory interactions between PrfA and sigma(B). The emerging model of the role of sigma(B) in regulating overall PrfA activity includes a switch from transcriptional activation at the P2(prfA) promoter (e. g., in extracellular bacteria when PrfA activity is low) to posttranscriptional downregulation of PrfA regulon expression (e. g., in intracellular bacteria when PrfA activity is high).
引用
收藏
页码:2113 / 2124
页数:12
相关论文
共 75 条
[1]  
[Anonymous], CURR PROTOC MICROBIO
[2]   Identification of the gene encoding the alternative sigma factor σB from Listeria monocytogenes and its role in osmotolerance [J].
Becker, LA ;
Çetin, MS ;
Hutkins, RW ;
Benson, AK .
JOURNAL OF BACTERIOLOGY, 1998, 180 (17) :4547-4554
[3]   Regulation of hly expression in Listeria monocytogenes by carbon sources and pH occurs through separate mechanisms mediated by PrfA [J].
Behari, J ;
Youngman, P .
INFECTION AND IMMUNITY, 1998, 66 (08) :3635-3642
[4]   Microarray-based analysis of the Staphylococcus aureus σB regulon [J].
Bischoff, M ;
Dunman, P ;
Kormanec, J ;
Macapagal, D ;
Murphy, E ;
Mounts, W ;
Berger-Bächi, B ;
Projan, S .
JOURNAL OF BACTERIOLOGY, 2004, 186 (13) :4085-4099
[5]  
BISHOP DK, 1987, J IMMUNOL, V139, P2005
[6]   Differential regulation of the virulence genes of Listeria monocytogenes by the transcriptional activator PrfA [J].
Bohne, J ;
Kestler, H ;
Uebele, C ;
Sokolovic, Z ;
Goebel, W .
MOLECULAR MICROBIOLOGY, 1996, 20 (06) :1189-1198
[7]   The bvr locus of Listeria monocytogenes mediates virulence gene repression by β-glucosides [J].
Brehm, K ;
Ripio, MT ;
Kreft, J ;
Vázquez-Boland, JA .
JOURNAL OF BACTERIOLOGY, 1999, 181 (16) :5024-5032
[8]   The Listeria monocytogenes iap gene as an indicator gene for the study of PrfA-dependent regulation [J].
Bubert, A ;
Kestler, H ;
Gotz, M ;
Bockmann, R ;
Goebel, W .
MOLECULAR & GENERAL GENETICS, 1997, 256 (01) :54-62
[9]   DUAL ROLES OF PLCA IN LISTERIA-MONOCYTOGENES PATHOGENESIS [J].
CAMILLI, A ;
TILNEY, LG ;
PORTNOY, DA .
MOLECULAR MICROBIOLOGY, 1993, 8 (01) :143-157
[10]   Microarray-Based characterization of the Listeria monocytogenes cold regulon in log- and stationary-phase cells [J].
Chan, Yvonne C. ;
Raengpradub, Sarita ;
Boor, Kathryn J. ;
Wiedmann, Martin .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (20) :6484-6498