Avidin conjugation to up-conversion phosphor NaYF4:Yb3+, Er3+ by the oxidation of the oligosaccharide chains

被引：16

作者：

Kong, Deyan ^{[1
,2
]}

Quan, Zewei ^{[1
]}

Yang, Jun ^{[1
]}

Yang, Piaoping ^{[1
]}

Li, Chunxia ^{[1
]}

Lin, Jun ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Rare Earth Resource Utilizat, Changchun 130022, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

来源：

JOURNAL OF NANOPARTICLE RESEARCH | 2009年 / 11卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Up-conversion phosphor; Avidin; Oligosaccharide chains; Fluorescence probe; Nanoparticles; Biomolecules; NANOSCALE FUNCTIONAL MATERIALS; POLYOL-MEDIATED SYNTHESIS; COLLOIDAL PARTICLES; QUANTUM DOTS; SIZE RANGE; NANOPARTICLES; NANOCRYSTALS; SILICA; IMMOBILIZATION; FLUORESCENCE;

D O I：

10.1007/s11051-008-9437-5

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

NaYF4:Yb3+, Er3+ nanoparticles were successfully prepared by a polyol process using diethyleneglycol (DEG) as solvent. After being functionalized with SiO2-NH2 layer, these NaYF4:Yb3+, Er3+ nanoparticles can conjugate with activated avidin molecules (activated by the oxidation of the oligosaccharide chain). The as-formed NaYF4:Yb3+, Er3+ nanoparticles, NaYF4:Yb3+, Er3+ nanoparticles functionalized with amino groups, avidin conjugated amino-functionalized NaYF4:Yb3+, Er3+ nanoparticles were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), atomic force microscopy (AFM), Fourier transform infrared (FT-IR), UV/Vis absorption spectra, and up-conversion luminescence spectra, respectively. The biofunctionalization of the NaYF4:Yb3+, Er3+ nanoparticles has less effect on their luminescence properties, i.e., they still show the up-conversion emission (from Er3+, with S-4(3/2) -> I-4(15/2) at similar to 540 nm and F-4(9/2) -> I-4(15/2) at similar to 653 nm), indicative of the great potential for these NaYF4:Yb3+, Er3+ nanoparticles to be used as fluorescence probes for biological system.

引用

页码：821 / 829

页数：9

共 41 条

[1]

Bayer E A, 1980, Methods Biochem Anal, V26, P1

[2]

Bellamy L.J., 1975, INFRA RED SPECTRA CO, VI

[3] PARTICLE SIZE DETERMINATION FROM X-RAY LINE BROADENING [J].