Apparent gas-phase acidities of multiply protonated peptide ions: Ubiquitin, insulin B, and renin substrate

被引:64
作者
Zhang, X [1 ]
Cassady, CJ [1 ]
机构
[1] MIAMI UNIV,DEPT CHEM,OXFORD,OH 45056
关键词
D O I
10.1016/S1044-0305(96)00110-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The gas-phase deprotonation reactions of multiply protonated bovine ubiquitin, insulin chain B, and renin substrate tetradecapeptide ions have been studied in a Fourier transform ion cyclotron resonance mass spectrometer coupled with an external electrospray source. Rate constants were measured for the reactions of these peptide ions with a series of reference compounds of known gas-phase basicities ranging from 195.6 to 232.6 kcal/mol. The apparent gas-phase acidities (GA(app)) of the multiply protonated peptide ions [M + nH](n+) were determined with deprotonation reactions. The deduced values of GA(app) show a strong dependence on the charge states of the multiply protonated peptide ions. In general, the values decrease as the charge states of the peptide ions increase. For ubiquitin ions, the determined GA(app)s values decrease from > 232.6 to 205.0 kcal/mol for n = 4-13; for GA(app)s decrease from > 232.6 to 198.2 kcal/mol for n = 2-5; for renin insulin B ions, the GA(app)s decrease from > 232.6 to 198.2 kcal/mol for n = 2-5; for renin substrate ions, the GA(app)s decrease from 221.6 to < 195.6 kcal/mol for n = 2-4. Interestingly, at a given mass-to-charge ratio, the GA(app)s of these peptide ions agree within 10 kcal/mol despite large differences in their mass and charge. The ubiquitin and insulin B ions generated under the present conditions reveal multiple isomers at certain charge states, n = 4, 5, 6, 12 for ubiquitin and n = 4, 5 for insulin B, as evidenced by the fact that the isomers display distinctively different deprotonation reaction rates with certain reference compounds. (C) 1996 American Society for Mass Spectrometry.
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页码:1211 / 1218
页数:8
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