Inhibition of nitric oxide synthase does not prevent ocular dominance plasticity in kitten visual cortex

被引:39
作者
Ruthazer, ES
Gillespie, DC
Dawson, TM
Snyder, SH
Stryker, MP
机构
[1] UNIV CALIF SAN FRANCISCO,DEPT PHYSIOL,PROGRAM NEUROSCI,SAN FRANCISCO,CA 94143
[2] UNIV CALIF SAN FRANCISCO,KECK CTR INTEGRAT NEUROSCI,SAN FRANCISCO,CA 94143
[3] JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROSCI,BALTIMORE,MD 21205
[4] JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROL,BALTIMORE,MD 21205
[5] JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROSCI PHARMACOL & MOLEC SCI,BALTIMORE,MD 21205
[6] JOHNS HOPKINS UNIV,SCH MED,DEPT PSYCHIAT,BALTIMORE,MD 21205
来源
JOURNAL OF PHYSIOLOGY-LONDON | 1996年 / 494卷 / 02期
关键词
D O I
10.1113/jphysiol.1996.sp021510
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. The neural messenger molecule nitric oxide (NO) has been shown to be involved in several forms of plasticity including hippocampal long-term potentiation. We examined the effects of chronic intracortical infusion of inhibitors of NO synthase (NOS) activity on the plasticity of visual cortical responses following monocular lid suture during the critical period. 2. Single unit recordings (618 cells) made in both the NOS inhibitor-treated (30 nM N-G-methyl-L-arginine (L-NMMA), or 22 or 2 mM nitro-L-arginine (L-NOArg)) and saline-treated control hemispheres of barbiturate-anaesthetized, critical-period kittens (n = 8) revealed a profound shift in favour of the non-deprived eye. Shifts were of similar magnitude in hemispheres in which NOX was inhibited and in saline control hemispheres. 3. Subsequent analysis of NOS activity in the same cortical tissue in which recordings had been made showed a pronounced decrease in NOS activity in inhibitor-treated hemispheres. In the region in which all the single unit recordings were made (<3 mm from the infusion cannula), 22 mM L-NOArg resulted in a reduction of NOX activity to 5.55 +/- 5.33% of control hemisphere NOS activity levels. L-NOArg (2 mM) and L-NMMA (30 mM) also produced clear, but smaller, inhibition of NOS activity 4. These findings demonstrate that NOS activity is not essential for ocular dominance plasticity in visual cortex.
引用
收藏
页码:519 / 527
页数:9
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