Modulation of cholinergic locus expression by glucocorticoids and retinoic acid is cell-type specific

被引：39

作者：

Berse, B

Blusztajn, JK

机构：

[1] BOSTON UNIV, SCH MED, DEPT PATHOL, BOSTON, MA 02118 USA

[2] BOSTON UNIV, SCH MED, DEPT PSYCHIAT, BOSTON, MA 02118 USA

来源：

FEBS LETTERS | 1997年 / 410卷 / 2-3期

关键词：

choline acetyltransferase; vesicular acetylcholine transporter; glucocorticoid; retinoic acid; PC12; cell; SN56;

D O I：

10.1016/S0014-5793(97)00568-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Modulation of mRNA expression of choline acetyltransferase (ChAT) and the vesicular acetylcholine transporter (VAChT) by the glucocorticoid dexamethasone and by retinoic acid was examined in two neuronal cell lines: basal forebrain-derived SN56 and pheochromocytoma PC12. Dexamethasone up-regulated ChAT and VAChT in SN56 cells, while it had inhibitory effects on these genes in PC12 cells. Retinoic acid stimulated the cholinergic markers in both cell types, but in SN56 cells its effect was partially additive with that of dexamethasone, whereas it was much smaller and abrogated by dexamethasone in PC12 cells, Acetylcholine content correlated with these mRNA changes. The presence of a glucocorticoid response element consensus sequence in the VAChT/ChAT gene locus suggests direct transcriptional regulation by glucocorticoids. (C) 1997 Federation of European Biochemical Societies.

引用

页码：175 / 179

页数：5

共 41 条

[1] IMMUNOSUPPRESSION BY GLUCOCORTICOIDS - INHIBITION OF NF-KAPPA-B ACTIVITY THROUGH INDUCTION OF I-KAPPA-B SYNTHESIS [J].