Mucosal immunization with live recombinant bovine respiratory syncytial virus (BRSV) and recombinant BRSV lacking the envelope glycoprotein G protects against challenge with wild-type BRSV

被引：30

作者：

Schmidt, U

Beyer, J

Polster, U

Gershwin, LJ

Buchholz, UJ

机构：

[1] Fed Res Ctr Virus Dis Anim, Friedrich Loeffler Inst, Inst Mol Biol, D-17498 Insel Riems, Germany

[2] Fed Res Ctr Virus Dis Anim, Friedrich Loeffler Inst, Inst Infectol, D-17498 Insel Riems, Germany

[3] Univ Calif Davis, Sch Vet Med, Dept Pathol Microbiol & Immunol, Davis, CA 95616 USA

来源：

JOURNAL OF VIROLOGY | 2002年 / 76卷 / 23期

关键词：

D O I：

10.1128/JVI.76.23.12355-12359.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Recombinant bovine respiratory syncytial virus (rBRSV) and an rBRSV deletion mutant lacking the G gene (rBRSVAG) were characterized in calves with respect to replication competence, attenuation, and protective efficacy as live-attenuated BRSV vaccines. Both recombinant viruses were safe and induced protection against a BRSV challenge infection. rBRSV replicated efficiently in the upper respiratory tract. Intranasal immunization with rBRSVAG led to infection but not to mucosal virus replication. Neutralizing antibodies were induced by rBRSV and rBRSVAG. Thus, the BRSV attachment glycoprotein G seems to be dispensable in vaccinating calves against BRSV.

引用

页码：12355 / 12359

页数：5

共 38 条

[1]

BRYSON DG, 1983, AM J VET RES, V44, P1648

[2] Chimeric bovine respiratory syncytial virus with glycoprotein gene substitutions from human respiratory syncytial virus (HRSV): Effects on host range and evaluation as a live-attenuated HRSV vaccine [J].