Repression of apoC-III gene expression by TNF alpha involves C/EBP delta/NF-IL6 beta via an IL-1 independent pathway

被引：17

作者：

Lacorte, JM ^{[1
]}

Beigneux, A ^{[1
]}

Parant, M ^{[1
]}

Chambaz, J ^{[1
]}

机构：

[1] URA CNRS 1283,F-75006 PARIS,FRANCE

来源：

FEBS LETTERS | 1997年 / 415卷 / 02期

关键词：

apolipoprotein; transcription; tumor necrosis factor alpha; C/EBP delta;

D O I：

10.1016/S0014-5793(97)01127-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Apolipoproteins A-II and C-III, which participate in the control of cholesterolemia and triglyceridemia, are negative acute phase proteins, Treatment of HepG2 cells with TNF alpha showed that apoA-II and apoC-III mRNA levels were decreased, Using transient transfection, we found that apoC-III gene expression is controlled at the transcriptional level. By competition and supershift experiments, we demonstrate that TNF alpha-induced complexes mere related to C/EBP delta/NF-IL6 beta and p50 and that overexpression of C/EBP delta was able to reproduce the inhibitory effect of TNF alpha on the apoC-III promoter, RT-PCR failed to detect the IL-1 transcript in TNF alpha-treated HepG2 cells, suggesting that activation of C/EBP delta by TNF alpha is not related to the IL-1-signalling pathway. (C) 1997 Federation of European Biochemical Societies.

引用

页码：217 / 220

页数：4

共 36 条

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Aggarwal BB, 1996, EUR CYTOKINE NETW, V7, P93

[2] A NUCLEAR FACTOR FOR IL-6 EXPRESSION (NF-IL6) IS A MEMBER OF A C/EBP FAMILY [J].