Activation of diacylglycerol O-acyltransferase 1 gene results in increased tumor necrosis factor-α gene expression in 3T3-L1 adipocytes

被引：15

作者：

Hirata, Takashi

Unoki, Hiroyuki

Bujo, Hideaki

Ueno, Koichi

Saito, Yasushi

机构：

[1] Chiba Univ, Grad Sch Med, Dept Genome Res & Clin Appl, Chuo Ku, Chiba 2608670, Japan

[2] Chiba Univ, Grad Sch Med, Div Appl Translat Res, Chiba 2608670, Japan

[3] Chiba Univ, Grad Sch Pharmaceut Sci, Dept Geriatr Pharmacol & Therapeut, Chiba 2608675, Japan

[4] Chiba Univ, Grad Sch Med, Dept Clin Cell Biol, Chiba 2608670, Japan

来源：

FEBS LETTERS | 2006年 / 580卷 / 21期

关键词：

adipocytes; tumor necrosis factor-alpha; diacylglycerol O-acyltransferase 1; triglyceride synthesis; insulin resistance;

D O I：

10.1016/j.febslet.2006.08.047

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The tumor necrosis factor-alpha (TNF-alpha) expression has been reported to be largely dependent on the size of adipocytes. We herein investigated the gene regulation of diacylglycerol O-acyltransferase (DGAT) in order to clarify the mechanism of TNF-alpha expression induced in large adipocytes. 3T3-L1 cells were cultured in the presence of 5 mM or 25 mM glucose to generate adipocytes from which the triglyceride content differs. The expression of TNF-alpha, DGAT1, and DGAT2 were upregulated in adipocytes, cultured with 25 mM glucose. Furthermore, knockdown of DGAT1 gene significantly inhibited the TNF-alpha expression. Finally, the DGAT1 expression levels were closely related to the TNF-alpha level in 3T3-L1 adipocytes. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：5117 / 5121

页数：5

共 22 条

[1] Increased diacylglycerol acyltransferase activity is associated with triglyceride accumulation in tissues of diet-induced insulin-resistant hyperlipidemic hamsters [J].