NMDA receptor-mediated control of protein synthesis at developing synapses

被引:297
作者
Scheetz, AJ
Nairn, AC
Constantine-Paton, M
机构
[1] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
[2] Rockefeller Univ, Mol & Cellular Neurosci Lab, New York, NY 10021 USA
关键词
D O I
10.1038/72915
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We demonstrate a rapid and complex effect of N-methyl-D-aspartate receptor (NMDAR) activation on synaptic protein synthesis in the superior colliculi of young rats. Within minutes of receptor activation, translation of alpha Ca2+/calmodulin dependent kinase II (alpha CamK II) was increased, whereas total protein synthesis was reduced. NMDAR activation also increased phosphorylation of eukaryotic elongation factor 2 (eEF2), a process known to inhibit protein translation by reducing peptide chain elongation. Low doses of cycloheximide, which reduce elongation rate independently of eEF2 phosphorylation, decreased overall protein synthesis but increased alpha CaMK II synthesis. These observations suggest that regulation of peptide elongation via eEF2 phosphorylation can link NMDAR activation to local increases in the synthesis of specific proteins during activity-dependent synaptic change.
引用
收藏
页码:211 / 216
页数:6
相关论文
共 40 条
[1]   MULTIFUNCTIONAL CA-2+/CALMODULIN-DEPENDENT PROTEIN-KINASE IS NECESSARY FOR NUCLEAR-ENVELOPE BREAKDOWN [J].
BAITINGER, C ;
ALDERTON, J ;
POENIE, M ;
SCHULMAN, H ;
STEINHARDT, RA .
JOURNAL OF CELL BIOLOGY, 1990, 111 (05) :1763-1773
[2]  
BRENDLER T, 1981, J BIOL CHEM, V256, P1747
[3]  
BRENDLER T, 1981, J BIOL CHEM, V256, P1755
[4]  
BROCKE L, 1995, J NEUROSCI, V15, P6797
[5]  
BURGIN KE, 1990, J NEUROSCI, V10, P1788
[6]   PATTERNED ACTIVITY, SYNAPTIC CONVERGENCE, AND THE NMDA RECEPTOR IN DEVELOPING VISUAL PATHWAYS [J].
CONSTANTINEPATON, M ;
CLINE, HT ;
DEBSKI, E .
ANNUAL REVIEW OF NEUROSCIENCE, 1990, 13 :129-154
[7]   Molecular characterization of the dendritic growth cone: Regulated mRNA transport and local protein synthesis [J].
Crino, PB ;
Eberwine, J .
NEURON, 1996, 17 (06) :1173-1187
[8]  
FEIG S, 1993, J NEUROSCI, V13, P1010
[9]  
GODEFROYCOLBURN T, 1981, J BIOL CHEM, V256, P1762
[10]  
HOLLINGSWORTH EB, 1985, J NEUROSCI, V5, P2240