Characterization and osteogenic effects of mesenchymal stem cells on microbeads composed of hydroxyapatite nanoparticles/reconstituted collagen

被引：11

作者：

Jeng, Long-Bin ^{[1
,2
]}

Chung, Hui-Ying ^{[3
]}

Lin, Tsung-Min ^{[3
]}

Chen, Jyh-Ping ^{[4
]}

Chen, Yi-Ling ^{[5
]}

Lu, Yi-Lung ^{[4
]}

Wang, Yng-Jiin ^{[6
]}

Chang, Sophia Chia-Ning ^{[1
,7
]}

机构：

[1] China Med Univ, Sch Med, Taichung 404, Taiwan

[2] China Med Univ Hosp, Dept Surg, Taichung 404, Taiwan

[3] China Med Univ Hosp, Dept Med Res, Taichung 404, Taiwan

[4] Chang Gung Univ, Grad Inst Biochem & Biomed Engn, Tao Yuan 333, Taiwan

[5] Chang Gung Mem Hosp, Dept Dent, Tao Yuan 333, Taiwan

[6] Natl Yang Ming Univ, Inst Biomed Engn, Taipei 112, Taiwan

[7] China Med Univ Hosp, Dept Plast Surg, Taichung 404, Taiwan

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2009年 / 91A卷 / 03期

关键词：

reconstituted collagen; hydroxyapatite; nanoparticle; mesenchymal stem cells; BONE MORPHOGENETIC PROTEIN-2; DIFFERENTIATION IN-VITRO; CALCIUM-PHOSPHATE; PROGENITOR CELLS; GENE-THERAPY; MARROW; VIVO; REGENERATION; PROLIFERATION; NANOCRYSTALS;

D O I：

10.1002/jbm.a.32293

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Natural bone is comprised of nanosized blade-like crystals of hydroxyapatite grown in close contact with collagen (Cot) fibers. Characteristics of artificial bone tissue differ considerably with those of natural ones, mainly from the unusual self-organizing interaction between the apatite crystals and the proteic components. Nanoparticle spheres of hydroxyapatite (n-HA), dispersed in reconstituted fibrous Cot, were prepared in three weight ratios of 75:25, 65:35, and 50:50 (n-HA:Col). Bone marrow mesenchymal stem cells (MSCs) from rabbits were seeded and cultured on the n-HA/Col microbeads and characterized. n-HA were evenly distributed throughout the Cot matrix and aggregated to microbeads as determined by scanning electron microscopy. Electron and confocal microscopy showed that the MSCs spread and attached to microbeads via focal adhesions, while staining for F-actin and DNA revealed the presence of stress fibers. The phenotype of the MSCs in the flow cytometry was identified as CD11a-, CD44+, and CD90.1+. The optimal weight ratio is 65:35 for the normalized alkaline phosphatase activities. The transduced MSCs, engineered by replication-defective adenovirus to express the BMP-2 gene, demonstrated synergic osteogenic effects in the microbeads. MSCs are capable of proliferating and differentiating in appropriate combinations of n-HA/Col. Thus it is a promising composite for future clinical applications. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 91A: 886-893, 2009

引用

页码：886 / 893

页数：8

共 53 条

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