Stepwise surface regeneration of electrochemical immunosensors working on biocatalyzed precipitation

被引：8

作者：

Yoon, HC ^{[1
]}

Ko, JS ^{[1
]}

Yang, H ^{[1
]}

Kim, YT ^{[1
]}

机构：

[1] ETRI, Basic Res Lab, BioMEMS Grp, Taejon 305350, South Korea

来源：

ANALYST | 2002年 / 127卷 / 12期

关键词：

D O I：

10.1039/b210244g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new strategy of stepwise surface regeneration for electrochemical immunosensors, working on a biocatalyzed precipitation reaction, has been developed. The strategy is based on the combination of deposited product thin-film dissolution and bound-protein displacement reactions from the modified sensor surfaces. As a model system, surfaces functionalized with biotin groups and their affinity recognition/ displacement reactions with antibiotin antibody molecules were chosen and investigated for affinity-sensing and stepwise regeneration reactions.

引用

页码：1576 / 1579

页数：4

共 18 条

[1] Identification and optimization of regeneration conditions for affinity-based biosensor assays.: A multivariate cocktail approach
Andersson, K
Hämäläinen, M
Malmqvist, M
[J]. ANALYTICAL CHEMISTRY, 1999, 71 (13) : 2475 - 2481
[2] Regenerable biosensor platform: A total internal reflection fluorescence cell with electrochemical control
Asanov, AN
Wilson, WW
Odham, PB
[J]. ANALYTICAL CHEMISTRY, 1998, 70 (06) : 1156 - 1163
[3] Micro-systems in biomedical applications
Dario, P
Carrozza, MC
Benvenuto, A
Menciassi, A
[J]. JOURNAL OF MICROMECHANICS AND MICROENGINEERING, 2000, 10 (02) : 235 - 244
[4] Avidin: A natural bridge for quantum dot-antibody conjugates
Goldman, ER
Balighian, ED
Mattoussi, H
Kuno, MK
Mauro, JM
Tran, PT
Anderson, GP
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (22) : 6378 - 6382
[5] Electric manipulation of bioparticles and macromolecules on microfabricated electrodes
Huang, Y
Ewalt, KL
Tirado, M
Haigis, TR
Forster, A
Ackley, D
Heller, MJ
O'Connell, JP
Krihak, M
[J]. ANALYTICAL CHEMISTRY, 2001, 73 (07) : 1549 - 1559
[6] Interference-based detection of nucleic acid targets on optically coated silicon
Jenison, R
Yang, S
Haeberli, A
Polisky, B
[J]. NATURE BIOTECHNOLOGY, 2001, 19 (01) : 62 - 65
[7] Chronopotentiometry and Faradaic impedance spectroscopy as methods for signal transduction in immunosensors
Katz, E
Alfonta, L
Willner, I
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 2001, 76 (1-3): : 134 - 141
[8] Precipitation of an insoluble product on enzyme monolayer electrodes for biosensor applications: Characterization by faradaic impedance spectroscopy, cyclic voltammetry, and microgravimetric quartz crystal microbalance analyses
Patolsky, F
Zayats, M
Katz, E
Willner, I
[J]. ANALYTICAL CHEMISTRY, 1999, 71 (15) : 3171 - 3180
[9] Molecular recognition between genetically engineered streptavidin and surface-bound biotin
Pérez-Luna, VH
O'Brien, MJ
Opperman, KA
Hampton, PD
López, GP
Klumb, LA
Stayton, PS
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (27) : 6469 - 6478
[10] Micro total analysis systems. 1. Introduction, theory, and technology
Reyes, DR
Iossifidis, D
Auroux, PA
Manz, A
[J]. ANALYTICAL CHEMISTRY, 2002, 74 (12) : 2623 - 2636

← 1 2 →