A WEE1 homologue from Arabidopsis thaliana

被引：69

作者：

Sorrell, DA

Marchbank, A

McMahon, K

Dickinson, JR

Rogers, HJ

Francis, D

机构：

[1] Cardiff Univ, Sch Biosci, Cardiff CF10 3TL, S Glam, Wales

[2] Hannah Res Inst, Ayr KA6 5HL, Scotland

[3] Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England

来源：

PLANTA | 2002年 / 215卷 / 03期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Arabidopsis (cell cycle); cell cycle; cyclin-dependent kinase; Schizosaccharomyces; wee1;

D O I：

10.1007/s00425-002-0815-4

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Little is known about the genes that regulate cyclinB-Cdc2 complexes at the G2/M transition of the plant cell cycle although in yeast and animals cdc25 and wee1 are central regulators of cdc2. Here we describe the isolation. by reverse transcription polymerase chain reaction (RT-PCR). of a WEE1 cDNA (AtTWEE1) in Arabidopsis thaliana (L.) Heynh. Semi-quantitative RTPCR showed that AtWEE1 expression was confined to actively dividing regions of the plant. The overexpression of AtWEE1 in fission yeast (Schizosaccharomyces pombe) caused cells to arrest, and to grow but not divide. resulting in very elongated cells. Our data provide evidence for a functional WEE1 in A. thaliana.

引用

页码：518 / 522

页数：5

共 17 条

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