Use of suppressive subtractive hybridization and cDNA arrays to discover patterns of altered gene expression in the liver of dihydrotestosterone and 11-ketotestosterone exposed adult male largemouth bass (Micropterus salmoides)

被引：21

作者：

Blum, JL

Knoebl, I

Larkin, P

Kroll, KJ

Denslow, ND

机构：

[1] Univ Florida, Interdisciplinary Ctr Biotechnol Res, Gainesville, FL 32610 USA

[2] Univ Florida, Interdisciplinary Program Biomed Res, Dept Pharmacol, Gainesville, FL 32610 USA

[3] EcoArray Inc, Alachua, FL 32615 USA

来源：

MARINE ENVIRONMENTAL RESEARCH | 2004年 / 58卷 / 2-5期

关键词：

dihydrotestosterone; 11-ketotestosterone; cDNA array; biomarkers; SSAT;

D O I：

10.1016/j.marenvres.2004.03.046

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

In this study male largemouth bass (LMB) were exposed to the naturally occurring androgens, dihydrotestosterone (DHT) or 11-ketotestosterone (11-KT) in order to identify genes that are differentially regulated by these steroid hormones. Using subtractive hybridization on livers of fish treated with DHT against vehicle control, many novel LMB genes were cloned. These genes were added to our gene library and arrayed. Six genes were up-regulated and five were down-regulated by both androgens. But, each androgen also regulated specific genes. One gene that was identified as a potential androgen marker was spermidine-spermine-N-acetyltransferase that was up-regulated by both androgens. Determining which genes are responsive to natural androgens will help to identify biochemical pathways that are impacted. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：565 / 569

页数：5

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