Simple and sensitive high-performance liquid chromatographic method for the determination of mycophenolic acid in plasma

被引:32
作者
Na-Bangchang, K
Supasyndh, O
Supaporn, T
Banmairuroi, V
Karbwang, J
机构
[1] Mahidol Univ, Fac Trop Med, Clin Pharmacol Unit, Bangkok, Thailand
[2] Pramongkutklao Med Coll, Dept Med, Bangkok, Thailand
来源
JOURNAL OF CHROMATOGRAPHY B | 2000年 / 738卷 / 01期
关键词
mycophenolic acid;
D O I
10.1016/S0378-4347(99)00487-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A rapid, selective, sensitive, and reproducible reversed-phase HPLC procedure for the quantitative determination of mycophenolic acid (MPA) - an active plasma metabolite of the immunosuppressant mycophenolate mofetil (MMF) in plasma is described. The procedure involves one-step extraction of MPA and the internal standard, standard [RS-60461-000: (E)-6-[1,3-dihydro-4-(4-carboxy-butoxy)-6-methoxy-7-methyl-3-oxo-5-isobenzo-furanyl-4-methyl-4-hexenoic acid] with dichloromethane-dichloroethane (1:1, v/v) at acidic pH. Chromatographic separation consisted of the mobile phase [acetonitrile-0.05% phosphate buffer, pH 3.4 (45:55, v/v)] running through the column (Techopak-10 C-18) at flow-rate of 0.8 ml/min. Detection was at UV wavelength of 254 nm. The mean recoveries of MPA and the internal standard at concentrations of 0.1 and 20 mu g/ml were 89-98%, and 90-96%, respectively. The within-day coefficients of variation for MPA were 0.3-7.8% and the day-to-day coefficients of variation were 1.1-2.0%. The minimum detectable concentrations for both MPA and the internal standard in plasma were 0.005 mu g/ml. The method was found to be suitable for use in clinical pharmacokinetic study. (C) 2000 Elsevier Science BN. All rights reserved.
引用
收藏
页码:169 / 173
页数:5
相关论文
共 11 条
[1]  
ALLISON AC, 1993, CLIN TRANSPLANT, V7, P96
[2]   DETERMINATION OF MYCOPHENOLIC ACID AND ITS GLUCURONIDE METABOLITE IN PLASMA [J].
BOPP, RJ ;
SCHIRMER, RE ;
MEYERS, DB .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1972, 61 (11) :1750-+
[3]  
GAINER FE, 1990, J PHARM SCI, V59, P1157
[4]  
GIBALDI M, 1989, BIOPHARMACEUTICAL CL
[5]   BIOAVAILABILITY IMPROVEMENT OF MYCOPHENOLIC-ACID THROUGH AMINO ESTER DERIVATIZATION [J].
LEE, WA ;
GU, L ;
MIKSZTAL, AR ;
CHU, N ;
LEUNG, K ;
NELSON, PH .
PHARMACEUTICAL RESEARCH, 1990, 7 (02) :161-166
[6]  
MORRIS RE, 1993, J HEART LUNG TRANSPL, V12, pS275
[7]   A TURBIDIMETRIC BIOASSAY METHOD FOR DETERMINATION OF MYCOPHENOLIC ACID [J].
NOTO, T ;
HARADA, Y ;
KOYAMA, K .
JOURNAL OF ANTIBIOTICS, 1970, 23 (02) :96-+
[8]   DETERMINATION OF A NEW IMMUNOSUPPRESSANT, MYCOPHENOLATE MOFETIL, AND ITS ACTIVE METABOLITE, MYCOPHENOLIC-ACID, IN RAT AND HUMAN-BODY FLUIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
SUGIOKA, N ;
ODANI, H ;
OHTA, T ;
KISHIMOTO, H ;
YASUMURA, T ;
TAKADA, K .
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1994, 654 (02) :249-256
[9]   High-performance liquid chromatographic method for the determination of mycophenolate mofetil in human plasma [J].
Tsina, I ;
Kaloostian, M ;
Lee, R ;
Tarnowski, T ;
Wong, B .
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1996, 681 (02) :347-353
[10]  
Tsina I, 1996, J CHROMATOGR B, V675, P119