Evaluation of molecular-beacon, TaqMan, and fluorescence resonance energy transfer probes for detection of antibiotic resistance-conferring single nucleotide Polymorphisms in mixed Mycobacterium tuberculosis DNA extracts

被引：26

作者：

Yesilkaya, Hasan

Meacci, Francesca

Niemann, Stefan

Hillemann, Doris

Ruesch-Gerdes, Sabine

Barer, Michael R.

Andrew, Peter W. ^{[1
]}

Oggioni, Marco R.

机构：

[1] Univ Leicester, Dept Infect Immun & Inflammat, Leicester LE1 9HN, Leics, England

[2] Univ Siena, Policlin Scotte, Lab Microbiol Mol & Biotecnol, Dipartimento Biol Mol, I-53100 Siena, Italy

[3] Forschungszentrum, Natl Reference Ctr Mycobacteria, Borstel, Germany

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2006年 / 44卷 / 10期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1128/JCM.00225-06

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The ability of fluorescence resonance energy transfer, molecular-beacon, and TaqMan probes to detect single nucleotide polymorphism (SNP) in the presence of a wild-type allele was evaluated using drug resistance-conferring SNPs in mixed Mycobacterium tuberculosis DNA. It was found that both the absolute quantity and the ratio of allelles determine the detection sensitivity of the probe systems.

引用

页码：3826 / 3829

页数：4

共 18 条

[1]

[Anonymous], CLIN MICROBIOL INFEC

[2] Molecular characterization of isoniazid-resistant Mycobacterium tuberculosis clinical isolates in Lithuania [J].