The ribosome-associated inhibitor A reduces translation errors

被引：36

作者：

Agafonov, DE ^{[1
]}

Spirin, AS ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Prot Res, Pushchino 142290, Moscow Region, Russia

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 320卷 / 02期

基金：

俄罗斯基础研究基金会;

关键词：

ribosome; cell-free translation; ribosome-associated inhibitor; miscoding; stress response;

D O I：

10.1016/j.bbrc.2004.05.171

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently we have reported about a novel stress response protein (pY or RaiA) associated with Escherichia coli ribosomes that inhibits translation at the aminoacyl-tRNA binding stage. Here we show that leucine misincorporation during in vitro poly(U) translation is inhibited by this protein much stronger than the incorporation of phenylalanine. The miscoding counteraction by RaiA is especially strong at the concentrations of magnesium ions close to those observed in vivo and diminishes at higher magnesium concentrations. The results obtained suggest that the anti-miscoding activity of RaiA could be the main function of the protein, rather than the inhibition of translation. The role of the protein in adaptation of cells to environmental stress is discussed. (C) 2004 Published by Elsevier Inc.

引用

页码：354 / 358

页数：5

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