Proteomic analysis of the anterior cingulate cortex in the major psychiatric disorders: Evidence for disease-associated changes

被引:249
作者
Beasley, Clare L.
Pennington, Kyla
Behan, Aine
Wait, Robin
Dunn, Michael J.
Cotter, David [1 ]
机构
[1] Royal Coll Surgeons Ireland, Dept Psychiat, Dublin 2, Ireland
[2] Inst Psychiat, Dept Psychol Med, London, England
[3] Univ London Imperial Coll Sci Technol & Med, Div Rheumatol, London, England
[4] Univ Coll Dublin, UCD Conway Inst Biomol & Biomed Res, Proteome Res Ctr, Dublin 2, Ireland
基金
英国惠康基金;
关键词
bipolar disorder; 2-D gel electrophoresis; metabolic; postmortem; schizophrenia;
D O I
10.1002/pmic.200500069
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Abnormalities of the anterior cingulate cortex have previously been described in schizophrenia, major depressive disorder and bipolar disorder. In this study 2-DE was performed followed by, mass spectrometric sequencing to identify disease-specific protein changes within the anterior cingulate cortex in these psychiatric disorders. The 2-DE system comprised IPGs 4-7 and 6-9 in the first, IEF dimension and SDS-PAGE in the second dimension. Resultant protein spots were compared between control and disease groups. Statistical analysis indicated that 35 spots were differentially expressed in one or more groups. Proteins comprising 26 of these spots were identified by mass spectroscopy. These represented 19 distinct proteins; aconitate hydratase, malate dehydrogenase, fructose bisphosphate aldolase A, ATP synthase, succinyl CoA ketoacid transferase, carbonic anhydrase, alpha- and beta-tubulin, dihydropyrimidinase-related protein-1 and -2, neuronal protein 25, trypsin precursor, glutamate dehydrogenase, glutamine synthetase, sorcin, vacuolar ATPase, creatine kinase, albumin and guanine nucleotide binding protein beta subunit. All but three of these proteins have previously been associated with the major psychiatric disorders. These findings provide support for the view that cytoskeletal and mitochondrial dysfunction are important components of the neuropathology of the major psychiatric disorders.
引用
收藏
页码:3414 / 3425
页数:12
相关论文
共 69 条
[1]   Anterior cingulate, gyrus rectus, and orbitofrontal abnormalities in elderly depressed patients: An MRI-based parcellation of the prefrontal cortex [J].
Ballmaier, M ;
Toga, AW ;
Blanton, RE ;
Sowell, ER ;
Lavretsky, H ;
Peterson, J ;
Pham, D ;
Kumar, A .
AMERICAN JOURNAL OF PSYCHIATRY, 2004, 161 (01) :99-108
[2]   Glycogen synthase kinase-3β immunoreactivity is reduced in the prefrontal cortex in schizophrenia [J].
Beasley, C ;
Cotter, D ;
Khan, N ;
Pollard, C ;
Sheppard, P ;
Varndell, I ;
Lovestone, S ;
Anderton, B ;
Everall, I .
NEUROSCIENCE LETTERS, 2001, 302 (2-3) :117-120
[3]   Mitochondrial dysfunction in schizophrenia: a possible linkage to dopamine [J].
Ben-Shachar, D .
JOURNAL OF NEUROCHEMISTRY, 2002, 83 (06) :1241-1251
[4]   THE ANATOMY OF MELANCHOLIA - FOCAL ABNORMALITIES OF CEREBRAL BLOOD-FLOW IN MAJOR DEPRESSION [J].
BENCH, CJ ;
FRISTON, KJ ;
BROWN, RG ;
SCOTT, LC ;
FRACKOWIAK, RSJ ;
DOLAN, RJ .
PSYCHOLOGICAL MEDICINE, 1992, 22 (03) :607-615
[5]  
BENES FM, 1995, ARCH GEN PSYCHIAT, V52, P1015
[6]  
BENES FM, 1991, ARCH GEN PSYCHIAT, V48, P996
[7]   Emerging principles of altered neural circuitry in schizophrenia [J].
Benes, FM .
BRAIN RESEARCH REVIEWS, 2000, 31 (2-3) :251-269
[8]   The density of pyramidal and nonpyramidal neurons in anterior cingulate cortex of schizophrenic and bipolar subjects [J].
Benes, FM ;
Vincent, SL ;
Todtenkopf, M .
BIOLOGICAL PSYCHIATRY, 2001, 50 (06) :395-406
[9]   Increased anterior cingulate and caudate activity in bipolar mania [J].
Blumberg, HP ;
Stern, E ;
Martinez, D ;
Ricketts, S ;
de Asis, J ;
White, T ;
Epstein, J ;
McBride, PA ;
Eidelberg, D ;
Kocsis, JH ;
Silbersweig, DA .
BIOLOGICAL PSYCHIATRY, 2000, 48 (11) :1045-1052
[10]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3