Serum unconjugated bile acids as a test for intestinal bacterial overgrowth in dogs

被引:32
作者
Melgarejo, T
Williams, DA
O'Connell, NC
Setchell, KDR
机构
[1] Texas A&M Univ, Sch Vet Med, Dept Small Anim Med & Surg, Gastrointestinal Lab, College Stn, TX 77843 USA
[2] Childrens Hosp, Med Ctr, Dept Pediat, Clin Mass Spectrometry Ctr, Cincinnati, OH 45229 USA
关键词
bile acids; bacterial overgrowth; intestinal disease;
D O I
10.1023/A:1005493416946
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Small intestinal bacterial overgrowth (SIBO) has a high incidence in dogs and, as in humans, is difficult to diagnose. The aim of this study was to determine the diagnostic significance of serum unconjugated bile acid concentrations in dogs with bacterial overgrowth. Fasting sera were obtained from 23 dogs: 10 with culture-proven SIBO, 8 with indirectly diagnosed SIBO (normal pancreatic function but small intestinal disease associated with subnormal serum cobalamin and supranormal folate concentrations), and 5 healthy controls. Unconjugated bile acids were determined using gas chromatography-mass spectrometry after isolation by liquid-solid extraction and anion-exchange chromatography. Mean serum unconjugated bile acid concentrations were significantly elevated in dogs with SIBO (mean +/- SD: 0.91 +/- 1.03 mu mol/liter), and in dogs with indirectly diagnosed SIBO (2.11 +/- 2.20 mu mol/liter) compared to clinically healthy dogs (0.015 +/- 0.015 mu mol/liter, P < 0.005). Cholic acid was the predominant unconjugated bile acid in the serum of dogs with SIBO. In conclusion serum unconjugated bile acid concentrations of healthy dogs are significantly lower than reported values for humans, and this fraction represents a relatively small proportion (0-2.3%; mean 0.8%) of the total bile acids in dogs. Unconjugated bile acids increased 10- to 20-fold in dogs with SIBO indicating the clinical utility of serum unconjugated bile acids for diagnosis of intestinal bacterial overgrowth in dogs.
引用
收藏
页码:407 / 414
页数:8
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