δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters

被引：42

作者：

Moretti, MB ^{[1
]}

García, SC ^{[1
]}

Perotti, C ^{[1
]}

Batlle, A ^{[1
]}

Casas, A ^{[1
]}

机构：

[1] Univ Buenos Aires, FCEN, Ctr Invest Sobre Porfirinas & Porfirias, CONICET, RA-1428 Buenos Aires, DF, Argentina

来源：

BRITISH JOURNAL OF CANCER | 2002年 / 87卷 / 04期

关键词：

alpha-aminolevulinic acid; membrane transport; photodynamic therapy; BETA transporters;

D O I：

10.1038/sj.bjc.6600481

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

delta-aminolevulinic acid, the precursor of porphyrin biosynthesis has been used to induce the endogenous synthesis of the photosensitiser protoporphyrin IX for photodynamic therapy in the treatment of various tumours. The aim of this work was to characterise the delta-aminolevulinic acid transport system in the murine mammary adenocarcinoma cell line LM3 using C-14-delta- aminolevulinic acid, to finally improve delta-aminolevulinic acid incorporation in mammalian cells. Our results showed that delta-aminolevulinic acid is incorporated into these cells by two different mechanisms, passive diffusion which is important at the beginning of the incubation, and active transport. Specificity assays suggested that the transporter involved in delta-aminolevulinic acid incorporation is a BETA transporter, probably GAT-2. (C) 2002 Cancer Research UK.

引用

页码：471 / 474

页数：4

共 25 条

[1] Expression cloning and functional characterization of the kidney cortex high-affinity proton-coupled peptide transporter [J].