Efficient subtyping of Yersinia enterocolitica bioserotype 4/O:3 with pulsed-field gel electrophoresis

被引：42

作者：

Fredriksson-Ahomaa, M ^{[1
]}

Autio, T ^{[1
]}

Korkeala, H ^{[1
]}

机构：

[1] Univ Helsinki, Fac Vet Med, Dept Food & Environm Hyg, FIN-00014 Helsinki, Finland

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1999年 / 29卷 / 05期

关键词：

D O I：

10.1046/j.1365-2672.1999.00625.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The purpose of this study was to evaluate the efficiency of pulsed-field gel electrophoresis (PFGE) for differentiation of isolates of Yersinia enterocolitica bioserotype 4/O:3 recovered from pig tongues at retail level in the Helsinki area during October and November of 1996. NotI generated 15 different PFGE patterns for 128 isolates. The discriminatory index did not exceed 74% due to the presence of two predominant PFGE-types, NA1 (58/128) and NB1 (25/128). After preliminary investigations with 35 enzymes, ApaI, XbaI, XhoI and SpeI were chosen for further characterization of the isolates. The discriminatory index increased from 74% to 93% and the number of different genotypes from 15 to 30 when isolates with the same PFGE pattern with NotI were further characterized with ApaI and XhoI, indicating that PFGE can be an efficient technique for characterization of bioserotype 4/O:3.

引用

页码：308 / 312

页数：5

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