Recent developments in NAD(P)H regeneration for enzymatic reductions in one- and two-phase systems

被引：44

作者：

Eckstein, M

Daussmann, T

Kragl, U

机构：

[1] Univ Rostock, Dept Chem, D-18059 Rostock, Germany

[2] JFC Julich Fine Chem GmbH, D-52428 Julich, Germany

来源：

BIOCATALYSIS AND BIOTRANSFORMATION | 2004年 / 22卷 / 02期

关键词：

alcohol dehydrogenase; glucose dehydrogenase; ionic liquid; organic solvent; oxidoreductase; two phase system;

D O I：

10.1080/10242420410001692769

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This review discusses recent achievements in the field of cofactor regeneration for the nicotinamide cofactors NADH and NADPH. The examples discussed include alcohol dehydrogenases, formate dehydrogenase, glucose dehydrogenase and a hydrogenase. For the reaction either one-phase systems or two-phase systems in combination with an organic solvent are discussed. For the enantioselective reduction of 2-octanone to ( R )-2-octanol it could be shown that enzyme coupled NADPH regeneration with glucose dehydrogenase and glucose results in shorter reaction times and higher yields when compared to the substrate coupled regeneration with 2-propanol.

引用

页码：89 / 96

页数：8

共 52 条

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