Improvement in production and quality of gellan gum by Sphingomonas paucimobilis under high dissolved oxygen tension levels

被引：59

作者：

Banik, R. M. ^{[1
]}

Santhiagu, A. ^{[1
]}

机构：

[1] Banaras Hindu Univ, Inst Technol, Sch Biomed Engn, Varanasi 221005, Uttar Pradesh, India

来源：

BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 17期

关键词：

aeration; agitation; exopolysaccharide; gellan; Sphingomonas paucimobilis;

D O I：

10.1007/s10529-006-9098-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

The effect of agitation rate and dissolved oxygen tension (DOT) on growth and gellan production by Sphingomonas paucimobilis was studied. Higher cell growth of 5.4 g l(-1) was obtained at 700 rpm but maximum gellan (15 g l(-1)) was produced at 500 rpm. DOT levels above 20% had no effect on cell growth but gellan yield was increased to 23 g l(-1) with increase in DOT level to 100%. Higher DOT levels improved the viscosity and molecular weight of the polymer with change in acetate and glycerate content of the polymer.

引用

收藏

页码：1347 / 1350

页数：4

相关论文

共 16 条

[1]

A MODIFIED URONIC ACID CARBAZOLE REACTION [J].

BITTER, T ;

MUIR, HM .

ANALYTICAL BIOCHEMISTRY, 1962, 4 (04) :330-&

[2]

Rheological evaluation of Gelrite® in situ gels for ophthalmic use [J].

Carlfors, J ;

Edsman, K ;

Petersson, R ;

Jornving, K .

EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 1998, 6 (02) :113-119

[3]

DISCHE Z, 1948, J BIOL CHEM, V175, P595

[4]

Influence of fermentation hydrodynamics on gellan gum physico-chemical characteristics [J].

Dreveton, E ;

Monot, F ;

Lecourtier, J ;

Ballerini, D ;

Choplin, L .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1996, 82 (03) :272-276

[5]

FATTAH EA, 1996, DRUG DEV IND PHARM, V24, P541

[6]

The effect of agitation and aeration on the synthesis and molecular weight of gellan in batch cultures of Sphingomonas paucimobilis [J].

Giavasis, I ;

Harvey, LM ;

McNeil, B .

ENZYME AND MICROBIAL TECHNOLOGY, 2006, 38 (1-2) :101-108

[7]

Analysis of structure and function of gellans with different substitution patterns [J].

Jay, AJ ;

Colquhoun, IJ ;

Ridout, MJ ;

Brownsey, GJ ;

Morris, VJ ;

Fialho, AM ;

Leitao, JH ;

Sa-Correia, I .

CARBOHYDRATE POLYMERS, 1998, 35 (3-4) :179-188

[8]

GELRITE AS A GELLING AGENT IN MEDIA FOR THE GROWTH OF THERMOPHILIC MICROORGANISMS [J].

LIN, CC ;

CASIDA, LE .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1984, 47 (02) :427-429

[9]

TEMPERATURE PROFILES OF GELLAN GUM SYNTHESIS AND ACTIVITIES OF BIOSYNTHETIC-ENZYMES [J].

MARTINS, LO ;

SACORREIA, I .

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 1994, 20 :385-395

[10]

DETERMINATION OF ACETYL IN PECTIN AND IN ACETYLATED CARBOHYDRATE POLYMERS - HYDROXAMIC ACID REACTION [J].

MCCOMB, EA ;

MCCREADY, RM .

ANALYTICAL CHEMISTRY, 1957, 29 (05) :819-821