Hydrogel probe for iontophoresis drug delivery to the eye

被引:32
作者
Eljarrat-Binstock, E
Raiskup, F
Frucht-Pery, J
Domb, AJ [1 ]
机构
[1] Hebrew Univ Jerusalem, Sch Pharm, Dept Med Chem & Nat Prod, Fac Med, IL-91120 Jerusalem, Israel
[2] Hadassah Univ Hosp, Dept Ophthalmol, IL-91120 Jerusalem, Israel
关键词
iontophoresis; hydrogel; gentamicin; dexamethasone; transscleral;
D O I
10.1163/156856204323005271
中图分类号
R318 [生物医学工程];
学科分类号
0831 [生物医学工程];
摘要
The aim of this study was to evaluate the use of a solid hydrogel loaded with a drug solution as a probe for ejecting drugs to the eye upon application of low current iontophoresis. Hydroxyethyl methacrylate (HEMA), cross-linked with ethylene glycol dimethacrylate (EGDMA), and cross-linked arabinogalactan or dextran were prepared to form solid hydrogels. The hydrogels were examined for their mechanical suitability, absorption of drug solution and in vitro release properties when applying an iontophoretic current through the drug-loaded hydrogel into a solid-agar surface. Transconjunctival and transscleral iontophoresis of gentamicin sulfate was studied in healthy rabbits using drug-loaded disposable HEMA hydrogel disc probes. Gentamicin concentrations in different eye segments were assayed using a fluorescence polarization immunoassay. Preliminary corneal toxicity was examined in rabbits using a current intensity of 2.5 and 5.1 mA/cm(2) for 60 and 120 s. The most appropriate hydrogel is composed of HEMA, 2% EGDMA and 75% water. Iontophoresis onto agar gel was found indicative for the evaluation of iontophoretic activity of a hydrogel. Transscleral iontophoretic treatment resulted in high concentrations of drugs in the posterior segments of the eye. Application of iontophoresis onto the rabbit eye caused a reversible swelling of the cornea which lasted a few hours after application. Low current iontophoresis using drug-loaded hydrogel has a potential clinical value in obtaining high drug concentration at posterior segments of the eye.
引用
收藏
页码:397 / 413
页数:17
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