Multi-color two-photon scanning microscopy using a 16-channel photomultiplier

被引：1

作者：

Buehler, C ^{[1
]}

Kim, KH ^{[1
]}

Greuter, U ^{[1
]}

Schlumpf, N ^{[1
]}

So, PTC ^{[1
]}

机构：

[1] Paul Scherrer Inst, CH-5232 Villigen, Switzerland

来源：

MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES II | 2002年 / 4620卷

关键词：

spectral imaging; multi-photon microscopy; tissue spectroscopy;

D O I：

10.1117/12.470694

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Two-photon scanning microscopy has been successfully applied in studying tissue structures and biochemistry with subcellular spatial resolution. We developed a 16-channel two-photon scanning microscope with high-performance single photon counting readout electronics. The apparatus incorporates a mode-locked Ti:Sapphire laser, a scanning microscope, a spectrograph with multi-anode PMT (16 channels with similar to 7 to 10 nm spectral resolution each), and a 16-channel photon counting card (PhCC) with integrated high-speed data link (payload 1.2 Gbps). Each PhCC detection channel features single-photon sensitivity and 100 MHz photon counting bandwidth. The multicolor single-photon counting detection scheme allows ultra-sensitive measurements in a broad spectrum of biomedical applications. We present spectroscopic studies of ex vivo tissue autofluorescence.

引用

页码：217 / 230

页数：14

共 20 条

[1] Rapid analysis of Raman image data using two-way multivariate curve resolution [J].