Heterogeneous shedding of Brucella abortus in milk and its effect on the control of animal brucellosis

被引:36
作者
Capparelli, R. [2 ]
Parlato, M.
Iannaccone, M.
Roperto, S. [3 ]
Marabelli, R. [4 ]
Roperto, F. [3 ]
Iannelli, D. [1 ]
机构
[1] Univ Naples Federico II, Cattedra Immunol, Fac Agr, I-80055 Naples, Italy
[2] Univ Naples Federico II, Fac Bictechnol Sci, I-80055 Naples, Italy
[3] Univ Naples Federico II, Fac Vet Med, I-80055 Naples, Italy
[4] Minist Hlth, Dept Vet Publ Hlth Nutr & Food Safety, Rome, Italy
关键词
pathogen transmission heterogeneity; quantitative PCR; superspreaders; QUANTITATIVE RISK-ASSESSMENT; ESCHERICHIA-COLI O157; BOVINE BRUCELLOSIS; INFECTION; CATTLE; PCR; TRANSMISSION; DIAGNOSIS; DYNAMICS; BLOOD;
D O I
10.1111/j.1365-2672.2009.04177.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aims: To ascertain whether in Brucella abortus-infected water buffalo herds, the number of newly infected animals could be reduced by culling superspreaders (the animals secreting >= 10(4) CFU per ml of milk). Methods and Results: The number of B. abortus present in the milk (CFU per nil) from 500 water buffaloes was measured by the Culture. Each animal was tested three times, at one month intervals. The presence or the absence of B. abortus in each milk sample was confirmed by PCR. A majority of infected animals shed the pathogen at a low level (<= 10(3) CFU ml(-1)). However, a few infected individuals (superspreaders) shed large numbers of B. abortus (>= 1(0)4 CFU ml(-1)). Quantitative PCR of B. abortus positive milk samples gave comparable results to Culture. Culling of the superspreaders was sufficient to arrest the spread of infection. Significance and Impact of the Study: The approach described here can reduce significantly the cost of controlling brucellosis. Culture and quantitative PCR tests identify superspreaders and, compared with the serological tests in use to detect brucellosis, provide also a more accurate estimate of the disease incidence.
引用
收藏
页码:2041 / 2047
页数:7
相关论文
共 29 条
  • [1] EVALUATION OF ALLERGIC AND SEROLOGICAL TESTS FOR DIAGNOSING BRUCELLA-MELITENSIS INFECTION IN SHEEP
    BLASCO, JM
    MARIN, C
    DEBAGUES, MJ
    BARBERAN, M
    HERNANDEZ, A
    MOLINA, L
    VELASCO, J
    DIAZ, R
    MORIYON, I
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (08) : 1835 - 1840
  • [2] Genetic resistance to Brucella abortus in the water buffalo (Bubalus bubalis)
    Borriello, G
    Capparelli, R
    Bianco, M
    Fenizia, D
    Alfano, F
    Capuano, F
    Ercolini, D
    Parisi, A
    Roperto, S
    Iannelli, D
    [J]. INFECTION AND IMMUNITY, 2006, 74 (04) : 2115 - 2120
  • [3] Brucellosis: a worldwide zoonosis
    Boschiroli, ML
    Foulongne, V
    O'Callaghan, D
    [J]. CURRENT OPINION IN MICROBIOLOGY, 2001, 4 (01) : 58 - 64
  • [4] Mannose-binding lectin haplotypes influence Brucella abortus infection in the water buffalo (Bubalus bubalis)
    Capparelli, R.
    Parlato, M.
    Amoroso, M. G.
    Roperto, S.
    Marabelli, R.
    Roperto, F.
    Iannelli, D.
    [J]. IMMUNOGENETICS, 2008, 60 (3-4) : 157 - 165
  • [5] Protective effifect of the Nramp1 BB genotype against Brucella abortus in the water buffalo (Bubalus bubalis)
    Capparelli, Rosanna
    Alfano, Flora
    Amoroso, Maria Grazia
    Borriello, Giorgia
    Fenizia, Domenico
    Bianco, Antonio
    Roperto, Sante
    Roperto, Franco
    Iannelli, Domenico
    [J]. INFECTION AND IMMUNITY, 2007, 75 (02) : 988 - 996
  • [6] Quantitative risk assessment for Escherichia coli O157:H7 in ground beef hamburgers
    Cassin, MH
    Lammerding, AM
    Todd, ECD
    Ross, W
    McColl, RS
    [J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1998, 41 (01) : 21 - 44
  • [7] Cheville NF, 1998, Brucellosis in the Greater Yellowstone Area
  • [8] Implantable cardioverter-defibrillator infection due to Brucella melitensis:: Case report and review of brucellosis of cardiac devices
    Dhand, Abhay
    Ross, John J.
    [J]. CLINICAL INFECTIOUS DISEASES, 2007, 44 (04) : E37 - E39
  • [9] The population dynamics of brucellosis in the Yellowstone National Park
    Dobson, A
    Meagher, M
    [J]. ECOLOGY, 1996, 77 (04) : 1026 - 1036
  • [10] Gall D, 2004, REV SCI TECH OIE, V23, P989