Cdc42 and mDia3 regulate microtubule attachment to kinetochores

被引:153
作者
Yasuda, S
Oceguera-Yanez, F
Kato, T
Okamoto, M
Yonemura, S
Terada, Y
Ishizaki, T
Narumiya, S [1 ]
机构
[1] Kyoto Univ, Fac Med, Dept Pharmacol, Kyoto 6068501, Japan
[2] Kyoto Univ, Fac Med, Horizontal Med Res Org, Kyoto 6068501, Japan
[3] RIKEN, Lab Cellular Morphogenesis, Ctr Dev Biol, Kobe, Hyogo 6500047, Japan
[4] Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA
关键词
D O I
10.1038/nature02452
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
During mitosis, the mitotic spindle, a bipolar structure composed of microtubules (MTs) and associated motor proteins(1,2), segregates sister chromatids to daughter cells. Initially some MTs emanating from one centrosome attach to the kinetochore at the centromere of one of the duplicated chromosomes. This attachment allows rapid poleward movement of the bound chromosome. Subsequent attachment of the sister kinetochore to MTs growing from the other centrosome results in the bi-orientation of the chromosome, in which interactions between kinetochores and the plus ends of MTs are formed and stabilized(2). These processes ensure alignment of chromosomes during metaphase and their correct segregation during anaphase. Although many proteins constituting the kinetochore have been identified and extensively studied, the signalling responsible for MT capture and stabilization is unclear(1,2). Small GTPases of the Rho family regulate cell morphogenesis by organizing the actin cytoskeleton and regulating MT alignment and stabilization(3). We now show that one member of this family, Cdc42, and its effector, mDia3, regulate MT attachment to kinetochores.
引用
收藏
页码:767 / 771
页数:5
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