The front-end logistics of DNA barcoding: challenges and prospects

被引：46

作者：

Borisenko, Alex V. ^{[1
]}

Sones, Jayme E. ^{[1
]}

Hebert, Paul D. N. ^{[1
]}

机构：

[1] Univ Guelph, Canadian Ctr DNA Barcoding, Biodivers Inst Ontario, Guelph, ON N1G 2W1, Canada

来源：

MOLECULAR ECOLOGY RESOURCES | 2009年 / 9卷

基金：

加拿大自然科学与工程研究理事会; 加拿大创新基金会;

关键词：

biodiversity; biomaterials; collection management; high-throughput sequencing; natural history; taxonomy; NATURAL-HISTORY MUSEUMS; COLLECTIONS; SPECIMEN; PROTOCOL; LIFE;

D O I：

10.1111/j.1755-0998.2009.02629.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Building a global library of DNA barcodes will require efficient logistics of pre-laboratory specimen processing and seamless interfacing with molecular protocols. If not addressed properly, the task of aggregating specimens may become the biggest bottleneck in the analytical chain. Three years of experience in developing a collection management system to facilitate high-throughput DNA barcoding have allowed the Canadian Centre for DNA Barcoding to recognize and resolve the most common logistical obstacles. Dealing with these challenges on a larger scale will be an important step towards building a solid collection-based foundation for the international DNA barcoding effort.

引用

页码：27 / 34

页数：8

共 34 条

[1] DNA barcoding in surveys of small mammal communities: a field study in Suriname [J].

Borisenko, Alex V. ;

Lim, Burton K. ;

Ivanova, Natalia V. ;

Hanner, Robert H. ;

Hebert, Paul D. N. .

MOLECULAR ECOLOGY RESOURCES, 2008, 8 (03) :471-479

[2] Natural history collections in crisis as funding is slashed [J].

Dalton, R .

NATURE, 2003, 423 (6940) :575-575

[3] The expansion of conservation genetics [J].

DeSalle, R ;

Amato, G .

NATURE REVIEWS GENETICS, 2004, 5 (09) :702-712

[4]

DeWaard Jeremy R., 2008, V410, P275

[5] The UK Biobank sample handling and storage protocol for the collection, processing and archiving of human blood and urine [J].

Elliott, Paul ;

Peakman, Tim C. .

INTERNATIONAL JOURNAL OF EPIDEMIOLOGY, 2008, 37 (02) :234-244

[6]

Ellis Rebecca., 2008, Museum and Society, V6, P172

[7]

Engstrom MD., 1999, Managing the Modern Herbarium: An Interdisciplinary Approach, P315

[8] Four years of DNA barcoding: Current advances and prospects [J].

Frezal, Lise ;

Leblois, Raphael .

INFECTION GENETICS AND EVOLUTION, 2008, 8 (05) :727-736

[9] DNA barcoding does not compete with taxonomy [J].

Gregory, TR .

NATURE, 2005, 434 (7037) :1067-1067

[10]

Gropp RE, 2003, BIOSCIENCE, V53, P550, DOI 10.1641/0006-3568(2003)053[0550:AUNSCG]2.0.CO

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