Phage display of combinatorial antibody libraries

被引：166

作者：

Rader, C ^{[1
]}

Barbas, CF ^{[1
]}

机构：

[1] Scripps Res Inst, DEPT MOL BIOL, LA JOLLA, CA 92037 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 1997年 / 8卷 / 04期

关键词：

D O I：

10.1016/S0958-1669(97)80075-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The selection of antibodies from combinatorial libraries displayed on the surface of filamentous phage has become an important methodology for the generation of reagent, diagnostic, and therapeutic molecules and for the study of natural immune responses. Using this technique, antibody genes have been cloned from multiple species or expressed directly from large man-made repertoires of antibody-encoding genes. Recent studies demonstrate that the technique allows for the in vitro evolution of antibodies to create molecules whose affinity for antigen exceeds that observed in nature.

引用

页码：503 / 508

页数：6

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