Extraction of amylase from fermentation broth in poly (ethylene glycol) salt aqueous two-phase system

被引：26

作者：

Bezerra, Raquel Pedrosa

Borbal, Fernanda Katharine Sousa Lins

Moreira, Keila Aparecida

Lima, Jose Luis, Jr.

Porto, Ana Lucia Figueiredo

Chaves, Adilson Castro

机构：

[1] Univ Fed Pernambuco, Inst Ciencias Biol, BR-50100010 Recife, PE, Brazil

[2] Univ Fed Pernambuco, Lab Imunol Keizo Asami, BR-50670901 Recife, PE, Brazil

[3] Univ Fed Pernambuco, Ctr Ciencias Biol, Doutorado Ciencias Biol, BR-50670901 Recife, PE, Brazil

[4] Univ Fed Pernambuco, Dept Morfol & Fisiol Anim, BR-52171900 Recife, PE, Brazil

[5] Fac Formacao Prof Nazare Mata, Lab Biotecnol, BR-55800000 Nazare Da Mata, PE, Brazil

[6] FUNESO, Fundacao Ensino Super Olinda, Uniao Escolas Super, BR-53060770 Olinda, PE, Brazil

[7] Univ Fed Pernambuco, Inst Ciencias Biol, Dept Patol, BR-50100010 Recife, PE, Brazil

来源：

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY | 2006年 / 49卷 / 04期

关键词：

amylases; aqueous two-phase systems; Bacillus subtilis; partition; poly (ethylene glycol);

D O I：

10.1590/S1516-89132006000500003

中图分类号：

Q [生物科学];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学];

摘要：

Studies were carried out on the partition of amylase from Bacillus subtilis in a minimal medium at 37 degrees C and 110 rpm. Enzyme recovery was carried out in aqueous two-phase system PEG-Phosphate salt were carried out. The best purification factor (5.4) was obtained in system PEG 1000 (16.7% w/w) with potassium phosphate (14.8% w/w), at pH 6.0, resulting in a recovery of 45.2% activity enzymatic in the salt-rich phase.

引用

页码：547 / 555

页数：9

共 33 条

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