The intracellular dissipation of cytosolic calcium following glucose re-addition to carbohydrate depleted Saccharomyces cerevisiae

被引：13

作者：

Kellermayer, R

Szigeti, R

Kellermayer, M

Miseta, A

机构：

[1] Univ Pecs, Dept Med Genet & Child Dev Med, H-7623 Pecs, Hungary

[2] Baranya Cty Hosp, Cent Lab, Pecs, Hungary

[3] Univ Pecs, Dept Clin Chem, Pecs, Hungary

来源：

FEBS LETTERS | 2004年 / 571卷 / 1-3期

基金：

匈牙利科学研究基金会;

关键词：

yeast; glucose; calcium; VCX1; PMR1; pH;

D O I：

10.1016/j.febslet.2004.06.049

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glucose re-addition to carbohydrate starved yeast cells leads to a transient elevation of cytosolic calcium (TECC). Concomitantly, a cytosolic proton extrusion occurs through the activation of the vacuolar H+-ATPase and the plasma membrane H+-ATPases. This study addressed the dissipation of the TECC through intracellular compartmentalization and the possible affects of the H+-ATPases on this process. Both the vacuole and the Golgi-ER apparatus were found to play important roles in distributing calcium to internal stores. Additionally, the inhibition of cytosolic proton extrusion augmented cytosolic calcium responses. A model where pH dependent cytosolic calcium buffering plays an important role in the dissipation of the TECC in Saccharomyces cerevisiae is proposed. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

收藏

页码：55 / 60

页数：6

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