Heme Oxygenase 1 Determines Atherosclerotic Lesion Progression Into a Vulnerable Plaque

被引:132
作者
Cheng, Caroline [1 ]
Noordeloos, Annemarie M. [1 ]
Jeney, Viktoria [4 ]
Soares, Miguel P. [4 ]
Moll, Frans [2 ]
Pasterkamp, Gerard [3 ]
Serruys, Patrick W. [1 ]
Duckers, Henricus J. [1 ]
机构
[1] Erasmus Univ, Med Ctr, Thoraxctr, Mol Cardiol Lab, NL-3015 CE Rotterdam, Netherlands
[2] Univ Med Ctr Utrecht, Dept Vasc Surg, Utrecht, Netherlands
[3] Univ Med Ctr Utrecht, Dept Cardiol, Utrecht, Netherlands
[4] Inst Gulbenkian Ciencias, Inflammat Lab, Oeiras, Portugal
关键词
atherosclerosis; coronary disease; inflammation; genes; vasculature; SMOOTH-MUSCLE-CELLS; SHEAR-STRESS; GENE-EXPRESSION; CIGARETTE-SMOKE; MEDIATED DEATH; MICE; MACROPHAGES; RUPTURE; PROTEIN; ARTERY;
D O I
10.1161/CIRCULATIONAHA.108.808618
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background-The molecular regulation for the transition from stable to vulnerable plaque remains to be elucidated. Heme oxygenase 1 (HO-1) and its metabolites have been implicated in the cytoprotective defense against oxidative injury in atherogenesis. In this study, we sought to assess the role of HO-1 in the progression toward plaque instability in carotid artery disease in patients and in a murine model of vulnerable plaque development. Methods and Results-Atherectomy biopsy from 112 patients with clinical carotid artery disease was collected and stratified according to characteristics of plaque vulnerability. HO-1 expression correlated closely with features of vulnerable human atheromatous plaque (P<0.005), including macrophage and lipid accumulation, and was inversely correlated with intraplaque vascular smooth muscle cells and collagen deposition. HO-1 expression levels correlated with the plaque destabilizing factors matrix metalloproteinase-9, interleukin-8, and interleukin-6. Likewise, in a vulnerable plaque model using apolipoprotein E-/- mice, HO-1 expression was upregulated in vulnerable versus stable lesions. HO-1 induction by cobalt protoporphyrin impeded lesion progression into vulnerable plaques, indicated by a reduction in necrotic core size and intraplaque lipid accumulation, whereas cap thickness and vascular smooth muscle cells were increased. In contrast, inhibition of HO-1 by zinc protoporphyrin augmented plaque vulnerability. Plaque stabilizing was prominent after adenoviral transduction of HO-1 compared with sham virus-treated animals, providing proof that the observed effects on plaque vulnerability were HO-1 specific. Conclusions-Here we demonstrate in a well-defined patient group and a murine vulnerable plaque model that HO-1 induction reverses plaque progression from a vulnerable plaque to a more stable phenotype as part of a compensatory atheroprotective response. (Circulation. 2009; 119: 3017-3027.)
引用
收藏
页码:3017 / A9
页数:20
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