Synthesis of an unusual polar glycopeptidolipid in glucose-limited culture of Mycobacterium smegmatis

被引：41

作者：

Ojha, AK

Varma, S

Chatterji, D ^{[1
]}

机构：

[1] Indian Inst Sci, Mol Biophys Unit, Bangalore 560012, Karnataka, India

[2] Ctr Cellular & Mol Biol, Hyderabad 500007, Andhra Pradesh, India

来源：

MICROBIOLOGY-SGM | 2002年 / 148卷

关键词：

polar glycopeptidolipid; carbon starvation;

D O I：

10.1099/00221287-148-10-3039

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

There has been a general understanding that Mycobacterium smegmatis produces only apolar glycopeptidolipid (GPL), similar in structure to serovar non-specific GPL of Mycobacterium avium. In this study, synthesis of polar GPL in carbon-starved M. smegmatis is reported. Mass spectrometric analysis suggests the polar GPL to be a hyperglycosylated species. The earlier structural studies of polar GPLs from M. avium have invariably shown the presence of an oligosaccharide appendage to D-allo-Thr. However, a further chemical analysis using beta-elimination of the newly found polar GPL in M. smegmatis shows that the molecule still contains a monosaccharide at the D-allo-Thr, thus suggesting a new form of polar GPL.

引用

页码：3039 / 3048

页数：10

共 24 条

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