MtHAP2-1 is a key transcriptional regulator of symbiotic nodule development regulated by microRNA169 in Medicago truncatula

被引:366
作者
Combier, Jean-Philippe
Frugier, Florian
de Billy, Francoise
Boualem, Adnane
El-Yahyaoui, Fikri
Moreau, Sandra
Vernie, Tatiana
Ott, Thomas
Gamas, Pascal
Crespi, Martin
Niebel, Andreas [1 ]
机构
[1] CNRS, Inst Natl Rech, INRA, LIPM, F-31326 Castanet Tolosan, France
[2] CNRS, ISV, F-91198 Gif Sur Yvette, France
关键词
nodulation; microRNA; transcription factor; symbiosis; HAP2; Medicago;
D O I
10.1101/gad.402806
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In the model legume Medicago truncatula, we identified a new transcription factor of the CCAAT-binding family, MtHAP2-1, for which RNA interference (RNAi) and in situ hybridization experiments indicate a key role during nodule development, possibly by controlling nodule meristem function. We could also show that MtHAP2-1 is regulated by microRNA169, whose overexpression leads to the same nodule developmental block as MtHAP2-1 RNAi constructs. The complementary expression pattern of miR169 and MtHAP2-1 and the phenotype of miR169-resistant MtHAP2-1 nodules strongly suggest, in addition, that the miR169-mediated restriction of MtHAP2-1 expression to the nodule meristematic zone is essential for the differentiation of nodule cells.
引用
收藏
页码:3084 / 3088
页数:5
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