Varietal identification in Cichorium intybus L and determination of genetic purity of F-1 hybrid seed samples, based on RAPD markers

被引:31
作者
Bellamy, A
Vedel, F
Bannerot, H
机构
[1] UNIV PARIS 11,URA CNRS 1128,CTR RECH PLANTES,F-91405 ORSAY,FRANCE
[2] INRA,STN AMELIORAT PLANTES,F-78026 VERSAILLES,FRANCE
关键词
Cichorium intybus; witloof chicory; RAPD markers; seed samples purity; varietal identification;
D O I
10.1111/j.1439-0523.1996.tb00887.x
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Random amplified polymorphic DNA (RAPD) markers were used to distinguish between several Cichorium intybus genotypes, comprising four white witloof inbred lines, three red witloof experimental inbred lines and a number of F1 hybrids derived from two white parents. Amplification conditions and reproducibility of RAPD patterns were examined. Comparison of polymerase chain reaction (PCR) products obtained by using 100 10-mer arbitrary primers allowed identification of all the lines analysed. With several primers, we defined line-specific RAPD markers, while with others polymorphism was more extensive, revealing several RAPD markers for several lines. All the differences were confirmed both on individual heads and young seedlings for each genotype. Because of the Mendelian segregation of these molecular markers, this method was applied to evaluate the genetic purity of F1 hybrid seed samples.
引用
收藏
页码:128 / 132
页数:5
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