Bottlenecks in the expression and secretion of heterologous proteins in Bacillus subtilis

被引：194

作者：

Li, WF ^{[1
]}

Zhou, XX ^{[1
]}

Lu, P ^{[1
]}

机构：

[1] Zhejiang Univ, Anim Sci Coll, Key Lab Mol Anim Nutr, Minist Educ, Hangzhou 310029, Peoples R China

来源：

RESEARCH IN MICROBIOLOGY | 2004年 / 155卷 / 08期

关键词：

Bacillus subtilis; transcription; protein folding; translocation; proteolysis;

D O I：

10.1016/j.resmic.2004.05.002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

Bacillus subtilis is an alternative host for expression and secretion of heterologous proteins. However, low yields of protein production limit its use on a wide scale. The secretory pathway of proteins can be divided into three functional stages: the early stage, involving the synthesis of secretory pre-proteins, their interaction with chaperones and binding to the secretory translocase; the second stage, translocation across the cytoplasmic membrane; and the last stage, including removal of the signal peptide, protein refolding and passage through the cell wall. Five bottlenecks for expression and secretion of heterologous proteins are described in this review: transcription, protein folding, translocation, signal peptide processing and proteolysis. (C) 2004 Elsevier SAS. All rights reserved.

引用

页码：605 / 610

页数：6

共 48 条

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[2]

Bacillus subtilis can modulate its capacity and specificity for protein secretion through temporally controlled expression of the sipS gene for signal peptidase I [J].