Generation of a monoclonal antibody to a defined portion of the Helicobacter pylori vacuolating cytotoxin by DNA immunization

被引：23

作者：

Ulivieri, C

Burroni, D

Telford, JL

Baldari, CT

机构：

[1] IRIS BIOCINE,DEPT BIOL MOL,I-53100 SIENA,ITALY

[2] UNIV SIENA,DEPT EVOLUT BIOL,I-53100 SIENA,ITALY

来源：

JOURNAL OF BIOTECHNOLOGY | 1996年 / 51卷 / 02期

关键词：

DNA immunization; monoclonal antibody; Helicobacter pylori;

D O I：

10.1016/0168-1656(96)01610-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The capacity of mammalian cells to express proteins encoded by plasmid vectors injected as naked DNA has opened the possibility to induce an immune response against protein antigens by DNA vaccination. We have used DNA immunization to generate a strong antibody response to a defined portion of the Helicobacter pylori vacuolating cytotoxin. A high specific monoclonal antibody has been derived from the splenocytes of an immunized mouse. Our data show that DNA immunization offers the possibility to obtain monoclonal antibodies following a significant shortcut with respect to traditional immunization protocols.

引用

页码：191 / 194

页数：4

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