Artificial gravity as a countermeasure to microgravity: a pilot study examining the effects on knee extensor and plantar flexor muscle groups

被引:50
作者
Caiozzo, V. J. [1 ,2 ]
Haddad, F. [2 ]
Lee, S. [4 ]
Baker, M. [1 ]
Paloski, William [3 ]
Baldwin, K. M. [2 ]
机构
[1] Univ Calif Irvine, Coll Hlth Sci, Dept Orthopaed, Irvine, CA 92697 USA
[2] Univ Calif Irvine, Coll Hlth Sci, Dept Physiol & Biophys, Irvine, CA 92697 USA
[3] Univ Houston, Dept Hlth & Human Performance, Houston, TX USA
[4] NASA, Lyndon B Johnson Space Ctr, Wyle Labs, Human Performance Lab, Houston, TX 77058 USA
基金
美国国家航空航天局; 美国国家卫生研究院;
关键词
gene expression; muscle biopsy; messenger ribonucleic acid; anabolic/catabolic markers; SKELETAL-MUSCLE; RESISTANCE EXERCISE; BED REST; ORTHOSTATIC INTOLERANCE; RESPONSES; SPACEFLIGHT; EXPRESSION; TOLERANCE; ATROPHY; SIZE;
D O I
10.1152/japplphysiol.91130.2008
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Caiozzo VJ, Haddad F, Lee S, Baker M, Paloski W, Baldwin KM. Artificial gravity as a countermeasure to microgravity: a pilot study examining the effects on knee extensor and plantar flexor muscle groups. J Appl Physiol 107: 39-46, 2009. First published March 12, 2009; doi: 10.1152/japplphysiol.91130.2008.-The goal of this project was to examine the effects of artificial gravity (AG) on skeletal muscle strength and key anabolic/catabolic markers known to regulate muscle mass. Two groups of subjects were selected for study: 1) a 21 day-bed rest (BR) group (n = 7) and 2) an AG group (n = 8), which was subjected to 21 days of 6 degrees head-down tilt bed rest plus daily 1-h exposures to AG (2.5 G at the feet). Centrifugation was produced using a short-arm centrifuge with the foot plate similar to 220 cm from the center of rotation. The torque-velocity relationships of the knee extensors and plantar flexors of the ankle were determined pre- and posttreatment. Muscle biopsy samples obtained from the vastus lateralis and soleus muscles were used for a series of gene expression analyses (mRNA abundance) of key factors implicated in the anabolic vs. catabolic state of the muscle. Post/pre torque-velocity determinations revealed greater decrements in knee extensor performance in the BR vs. AG group (P < 0.04). The plantar flexors of the AG subjects actually demonstrated a net gain in the torque-velocity relationship, whereas in the BR group, the responses declined (AG vs. BR, P < 0.001). Muscle fiber cross-sectional area decreased by similar to 20% in the BR group, whereas no losses were evident in the AG group. RT-PCR analyses of muscle biopsy specimens demonstrated that markers of growth and cytoskeletal integrity were higher in the AG group, whereas catabolic markers were elevated in the BR group. Importantly, these patterns were seen in both muscles. We conclude that paradigms of AG have the potential to maintain the functional, biochemical, and structural homeostasis of skeletal muscle in the face of chronic unloading.
引用
收藏
页码:39 / 46
页数:8
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