Similarities of omega gliadins from Triticum urartu to those encoded on chromosome 1A of hexaploid wheat and evidence for their post-translational processing

被引：55

作者：

DuPont, FM ^{[1
]}

Vensel, W ^{[1
]}

Encarnacao, T ^{[1
]}

Chan, R ^{[1
]}

Kasarda, DD ^{[1
]}

机构：

[1] ARS, USDA, Albany, CA 94710 USA

来源：

THEORETICAL AND APPLIED GENETICS | 2004年 / 108卷 / 07期

关键词：

D O I：

10.1007/s00122-003-1565-9

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The omega-gliadins encoded on chromosome 1 of the A genome were purified from Triticum aestivum L. (2n=6x=42, AABBDD) cv. Butte86, nullisomic 1D-tetrasomic 1A of cv. Chinese Spring (CS N1DT1A), and the diploid T. urartu (2n=2x=14, AA). Reverse-phase high-performance liquid chromatography combined with sodium dodecyl sulfate-polyacrylamide gel electrophoresis of gliadin extracts from CS nullisomic-tetrasomic (NT) lines confirmed the assignment to chromosome 1A. The purified omega-gliadins were characterized by mass spectrometry and N-terminal sequencing. The 1A-encoded omega-gliadins were smaller than 1B- or 1D-encoded omega-gliadins. The N-terminal amino acid sequences for 1A omega-gliadin mature peptides were nearly identical to those for the T. urartu omega-gliadins and were more similar to 1D omega-gliadin sequences than to sequences for T. monococum omega-gliadins, barley C-hordeins, or rye omega-secalins. They diverged greatly from the N-terminal sequences for the 1B omega-gliadins. The data suggest that T. urartu is the A-genome donor, and that post-translational cleavage by an asparaginyl endoprotease produces those omega-gliadins with N-terminal sequences beginning with KEL.

引用

页码：1299 / 1308

页数：10

共 51 条

[1] Effect of heat shock during grain filling on the gluten protein composition of bread wheat [J].