Improved infiltration of stem cells on electrospun nanofibers

被引：90

作者：

Shabani, Iman ^{[2
,4
]}

Haddadi-Asl, Vahid ^{[2
]}

Seyedjafari, Ehsan ^{[3
,4
]}

Babaeijandaghi, Farshad ^{[4
,5
]}

Soleimani, Masoud ^{[1
]}

机构：

[1] Tarbiat Modares Univ, Dept Hematol, Fac Med Sci, Tehran, Iran

[2] Amirkabir Univ Technol, Dept Polymer Engn, Tehran, Iran

[3] Univ Tehran, Dept Biotechnol, Coll Sci, Tehran, Iran

[4] Stem Cell Technol Co Ltd, Dept Stem Cells & Tissue Engn, Tehran, Iran

[5] Univ Tehran Med Sci, Fac Med, Tehran, Iran

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 382卷 / 01期

关键词：

Electrospinning; Polyethersuffone; Stem cell; Nanofiber; Scaffold; Tissue engineering; Infiltration; Plasma treatment; CELLULAR INFILTRATION; SURFACE MODIFICATION; PLASMA TREATMENT; COLLAGEN; DIFFERENTIATION; IMMOBILIZATION; SCAFFOLDS; GELATIN; GROWTH; FIBER;

D O I：

10.1016/j.bbrc.2009.02.150

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Nanofibrous scaffolds have been recently used in the field of tissue engineering because of their nano-size structure which promotes cell attachment, function, proliferation and infiltration. In this study, nanofibrous polyethersuffone (PES) scaffolds was prepared via electrospinning. The scaffolds were surface modified by Plasma treatment and collagen grafting. The surface changes then investigated by contact angle measurements and FTIR-ATR. The results proved grafting of the collagen on nanofibers surface and increased hydrophilicity after plasma treatment and collagen grafting. The cell interaction study was done using stem cells because of their ability to differentiate to different kinds of cell lines. The cells had normal morphology oil nanofibers and showed very high infiltration through collagen grafted PES nanofibers. This infiltration capability is very useful and needed to make 3D scaffolds in tissue engineering. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：129 / 133

页数：5

共 36 条

[1]

Acrylic acid grafting and collagen immobilization on poly(ethylene terephthalate) surfaces for adherence and growth of human bladder smooth muscle cells [J].