Alteration of gene expression in normal-appearing colon mucosa of APCmin mice and human cancer patients

被引：119

作者：

Chen, LC ^{[1
]}

Hao, CY ^{[1
]}

Chiu, YSY ^{[1
]}

Wong, P ^{[1
]}

Melnick, JS ^{[1
]}

Brotman, M ^{[1
]}

Moretto, J ^{[1
]}

Mendes, F ^{[1
]}

Smith, AP ^{[1
]}

Bennington, JL ^{[1
]}

Moore, D ^{[1
]}

Lee, NM ^{[1
]}

机构：

[1] Calif Pacific Med Ctr, Res Inst, San Francisco, CA 94115 USA

来源：

CANCER RESEARCH | 2004年 / 64卷 / 10期

关键词：

D O I：

10.1158/0008-5472.CAN-03-3264

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The expression of many genes is altered in colon cancer, but the roles of these genes in carcinogenesis are unclear. Using real-time quantitative PCR, we demonstrated that several genes previously implicated in human colon cancer undergo altered expression in the APC(min) mouse adenomatous polyp, a precursor of cancer, as well as in normal-appearing surrounding mucosa. The five genes that were most highly up-regulated in mouse polyp were also significantly up-regulated in polyp-free colon mucosa. Similar changes occurred in morphologically normal mucosa of surgical sections taken from human cancer patients, frequently extending to the margins. Thus, morphologically normal colon mucosa in APC(min) mice and in human cancer patients is not metabolically normal. Altered gene expression in this tissue does not appear to result from a field effect because there was no correlation between extent of altered regulation and distance from polyp or tumor. Our data suggest that alterations of expression levels of these genes may be an early event in carcinogenesis and a marker of risk for the development of colon cancer.

引用

页码：3694 / 3700

页数：7

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