The effects of elevated CO2 concentration on soybean gene expression.: An analysis of growing and mature leaves

被引:118
作者
Ainsworth, Elizabeth A. [1 ]
Rogers, Alistair
Vodkin, Lila O.
Walter, Achim
Schurr, Ulrich
机构
[1] Univ Illinois, USDA ARS, Photosynthesis Res Unit, Dept Plant Biol, Urbana, IL 61801 USA
[2] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA
[3] Brookhaven Natl Lab, Dept Environm Sci, Upton, NY 11973 USA
[4] Juelich Res Ctr, ICG 3, D-52425 Julich, Germany
关键词
D O I
10.1104/pp.106.086256
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Improvements in carbon assimilation and water-use efficiency lead to increases in maximum leaf area index at elevated carbon dioxide concentration ([CO2]); however, the molecular drivers for this increase are unknown. We investigated the molecular basis for changes in leaf development at elevated [CO2] using soybeans (Glycine max) grown under fully open air conditions at the Soybean Free Air CO2 Enrichment (SoyFACE) facility. The transcriptome responses of rapidly growing and fully expanded leaves to elevated [CO2] were investigated using cDNA microarrays. We identified 1,146 transcripts that showed a significant change in expression in growing versus fully expanded leaves. Transcripts for ribosomal proteins, cell cycle, and cell wall loosening, necessary for cytoplasmic growth and cell proliferation, were highly expressed in growing leaves. We further identified 139 transcripts with a significant [CO2] by development interaction. Clustering of these transcripts showed that transcripts involved in cell growth and cell proliferation were more highly expressed in growing leaves that developed at elevated [CO2] compared to growing leaves that developed at ambient [CO2]. The 327 [CO2]-responsive genes largely suggest that elevated [CO2] stimulates the respiratory breakdown of carbohydrates, which provides increased energy and biochemical precursors for leaf expansion and growth at elevated [CO2]. While increased photosynthesis and carbohydrate production at elevated [CO2] are well documented, this research demonstrates that at the transcript and metabolite level, respiratory breakdown of starch is also increased at elevated [CO2].
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页码:135 / 147
页数:13
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