Growth of osteoblast-like cells on porous hydroxyapatite ceramics:: an in vitro study

被引：110

作者：

Cerroni, L

Filocamo, R

Fabbri, M

Piconi, C

Caropreso, S

Condò, SG

机构：

[1] Univ Roma Tor Vergata, Dept Expt Med & Biochem Sci, I-00133 Rome, Italy

[2] FIN Ceram SRL, Faenza, Ravenna, Italy

[3] ENEA, New Mat Div, Rome, Italy

来源：

BIOMOLECULAR ENGINEERING | 2002年 / 19卷 / 2-6期

关键词：

osteoblasts; hydroxyapatite; porosity; aggregates;

D O I：

10.1016/S1389-0344(02)00027-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Blocks of two porous synthetic hydroxyapatites (HA) with porosity fraction of 30-40 and 50-60 vol%, respectively and a coralline derived porous HA were evaluated in vitro in presence of the osteogenic line MC3T3-E1 and of L929 fibroblasts. The two tested biomaterials did not affect cellular proliferation (MTT test), but the contact inhibited alkaline phosphatase activity. Porous aggregates resulted perfectly biocompatible in the tests performed, since observations performed by light microscopy did not show any cell morphological change, osteoblast presented a stellar shape and typical pseudopodes. SEM observations showed intercellular matrix containing fibers on HA-based porous aggregates. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：119 / 124

页数：6

共 27 条

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Allen Matthew, 1994, Clinical Materials, V16, P189

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