Tissue-specific regulation of IRS-2/PI 3-kinase association in aged rats

被引：6

作者：

Carvalho, CRO ^{[1
]}

Maeda, L ^{[1
]}

Brenelli, SL ^{[1
]}

Saad, MJA ^{[1
]}

机构：

[1] Univ Estadual Campinas, Fac Ciencias Med, Dept Clin Med, BR-13083970 Campinas, SP, Brazil

来源：

BIOLOGICAL CHEMISTRY | 2000年 / 381卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

aging; insulin action; insulin receptor substrate-2; insulin resistance; PI3-kinase; tyrosine phosphorylation;

D O I：

10.1515/BC.2000.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have examined the insulin-stimulated IRS-2 association with PI 3-kinase and the phosphorylation of AKT/PKB, which is functionally located downstream of the PI 3-kinase, in aged (obese) rats. The IRS-2 protein levels were similar in 2 and 20 month-old rats in both tissues, liver and muscle. There were reductions in insulin-induced IRS-2 tyrosine phosphorylation in liver and muscle, accompanied by a decrease in IRS-2/PI 3-kinase association and in AKT/PKB phosphorylation only in muscle tissue of aged rats. This regulation may be important in the altered glucose metabolism observed in aged (obese) rats.

引用

页码：75 / 78

页数：4

共 19 条

[1] PHOSPHATIDYLINOSITOL 3'-KINASE IS ACTIVATED BY ASSOCIATION WITH IRS-1 DURING INSULIN STIMULATION [J].

BACKER, JM ;

MYERS, MG ;

SHOELSON, SE ;

CHIN, DJ ;

SUN, XJ ;

MIRALPEIX, M ;

HU, P ;

MARGOLIS, B ;

SKOLNIK, EY ;

SCHLESSINGER, J ;

WHITE, MF .

EMBO JOURNAL, 1992, 11 (09) :3469-3479

[2] Effect of aging on insulin receptor, insulin receptor substrate-1, and phosphatidylinositol 3-kinase in liver and muscle of rats [J].

Carvalho, CRO ;

Brenelli, SL ;

Silva, AC ;

Nunes, ALB ;

Velloso, LA ;

Saad, MJA .

ENDOCRINOLOGY, 1996, 137 (01) :151-159

[3] INSULIN ACTION AND THE INSULIN SIGNALING NETWORK [J].

CHEATHAM, B ;

KAHN, CR .

ENDOCRINE REVIEWS, 1995, 16 (02) :117-142

[4]

CHEATHAM B, 1994, MOL BIOL CHEM, V265, P396

[5] The Tenth Datta Lecture - PDK1, one of the missing links in insulin signal transduction? [J].

Cohen, P ;

Alessi, DR ;

Cross, DAE .

FEBS LETTERS, 1997, 410 (01) :3-10

[6] Signal transduction - Lipid-regulated kinases: Some common themes at last [J].

Downward, J .

SCIENCE, 1998, 279 (5351) :673-674

[7] Insulin action and age [J].

Ferrannini, E ;

Vichi, S ;

BeckNielsen, H ;

Laakso, M ;

Paolisso, G ;

Smith, U .

DIABETES, 1996, 45 (07) :947-953

[8]

FINK RI, 1983, J CLIN INVEST, V71, P1523, DOI 10.1172/JCI110908

[9]

HARARI YR, 1995, J BIOL CHEM, V267, P17483

[10] AGING, EXERCISE AND FOOD RESTRICTION - EFFECTS ON SKELETAL-MUSCLE GLUCOSE-UPTAKE [J].

IVY, JL ;

YOUNG, JC ;

CRAIG, BW ;

KOHRT, WM ;

HOLLOSZY, JO .

MECHANISMS OF AGEING AND DEVELOPMENT, 1991, 61 (02) :123-133

← 1 2 →