Supramolecular organization of photosystem I and light- harvesting complex I in Chlamydomonas reinhardtii

被引:84
作者
Germano, M
Yakushevska, AE
Keegstra, W
van Gorkom, HJ
Dekker, JP
Boekema, EJ
机构
[1] Free Univ Amsterdam, Fac Sci, Dept Phys & Astron, NL-1081 HV Amsterdam, Netherlands
[2] Leiden Univ, Dept Biophys, Huygens Lab, NL-2300 RA Leiden, Netherlands
[3] Univ Groningen, Dept Biophys Chem, Groningen Biomol Sci & Biotechnol Inst, NL-9747 AG Groningen, Netherlands
来源
FEBS LETTERS | 2002年 / 525卷 / 1-3期
关键词
photosystem I; light-harvesting complex I; electron microscopy; state transitions; Chlamydomonas reinhardtii;
D O I
10.1016/S0014-5793(02)03100-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report a structural characterization by electron microscopy and image analysis of a supramolecular complex consisting of photosystem I and light-harvesting complex I from the unicellular green alga Chlamydomonas reinhardtii. The complex is a monomer, has longest dimensions of 21.3 and 18.2 nm in projection, and is significantly larger than the corresponding complex in spinach. Comparison with photosystem I complexes from other organisms suggests that the complex contains about 14 light-harvesting proteins, two or three of which bind at the side of the PSI-H subunit. We suggest that special light-harvesting I proteins play a role in the binding of phosphorylated light-harvesting complex II in state 2. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:121 / 125
页数:5
相关论文
共 31 条
  • [1] COPPER ENZYMES IN ISOLATED CHLOROPLASTS - POLYPHENOLOXIDASE IN BETA-VULGARIS
    ARNON, DI
    [J]. PLANT PHYSIOLOGY, 1949, 24 (01) : 1 - 15
  • [2] BASSI R, 1992, J BIOL CHEM, V267, P25714
  • [3] Oxyphotobacteria - Antenna ring around photosystem I
    Bibby, TS
    Nield, J
    Partensky, F
    Barber, J
    [J]. NATURE, 2001, 413 (6856) : 590 - 590
  • [4] Iron deficiency induces the formation of an antenna ring around trimeric photosystem I in cyanobacteria
    Bibby, TS
    Nield, J
    Barber, J
    [J]. NATURE, 2001, 412 (6848) : 743 - 745
  • [5] A giant chlorophyll-protein complex induced by iron defciency in cyanobacteria
    Boekema, EJ
    Hifney, A
    Yakushevska, AE
    Piotrowski, M
    Keegstra, W
    Berry, S
    Michel, KP
    Pistorius, EK
    Kruip, J
    [J]. NATURE, 2001, 412 (6848) : 745 - 748
  • [6] Green plant photosystem I binds light-harvesting complex I on one side of the complex
    Boekema, EJ
    Jensen, PE
    Schlodder, E
    van Breemen, JFL
    van Roon, H
    Scheller, HV
    Dekker, JP
    [J]. BIOCHEMISTRY, 2001, 40 (04) : 1029 - 1036
  • [7] Photosystem I: Function and physiology
    Chitnis, PR
    [J]. ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 2001, 52 : 593 - 626
  • [8] DEVITRY C, 1983, CR ACAD SCI III-VIE, V297, P277
  • [9] Structure of photosystem I
    Fromme, P
    Jordan, P
    Krauss, N
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2001, 1507 (1-3): : 5 - 31