On-line measurement of intramolecular carbon isotope distribution of acetic acid by continuous-flow isotope ratio mass spectrometry

被引:41
作者
Yamada, K [1 ]
Tanaka, M
Nakagawa, F
Yoshida, N
机构
[1] Tokyo Inst Technol, Interdisciplinary Grad Sch Sci & Engn, Dept Environm Chem & Engn, Yokohama, Kanagawa 2268502, Japan
[2] Japan Sci & Technol Corp, JST, CREST Project, Kawaguchi, Saitama 3320012, Japan
[3] Tokyo Inst Technol, Interdisciplinary Grad Sch Sci & Engn, Dept Environm Sci & Technol, Yokohama, Kanagawa 2268502, Japan
关键词
D O I
10.1002/rcm.678
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Molecular and intramolecular carbon isotope measurements of acetic acid present in natural environments have been performed by off-line procedures. The off-line method is complicated and time-consuming and requires micromolar to millimolar amounts of sample. This limits geochemical isotopic studies, especially at the intramolecular level, on acetic acid present in natural samples. Here, we examine an on-line measurement of intramolecular carbon isotope distribution of acetic acid using continuous-flow isotope ratio mass spectrometry (CF-IRMS) coupled with an on-line pyrolysis system. This is achieved by measurement of the respective carbon isotope ratios of CH4 and CO2 produced by on-line pyrolysis of acetic acid. Results for authentic standards of pure acetic acid demonstrated the practicality of this on-line method, although the carbon isotope ratio of the methyl group could not be determined directly. The precision of the carbon isotope measurements was 0.4parts per thousand (1sigma). The carbon isotope distribution determined by the on-line method was identical to that determined by the conventional off-line method within analytical error. The advantages of the online method compared with the conventional off-line method are that it is less laborious, requires less analytical time (less than one hour per sample) and, most importantly, uses smaller sample sizes (ca. 10 nanomole). An application of this on-line method to natural geochemical samples will provide an insight into the geochemical cycle of acetic acid. Copyright (C) 2002 John Wiley Sons, Ltd.
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页码:1059 / 1064
页数:6
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