Lipid modulatory activities of Cichorium glandulosum Boiss et Huet are mediated by multiple components within hepatocytes

被引:41
作者
Ding, Lin [1 ,2 ]
Liu, Jun-Lin [3 ]
Hassan, Waseem [1 ,2 ]
Wang, Lu-Lu [1 ,2 ]
Yan, Fang-Rong [3 ]
Shang, Jing [1 ,2 ]
机构
[1] China Pharmaceut Univ, Natl Ctr Drug Screening, Nanjing 210009, Jiangsu, Peoples R China
[2] China Pharmaceut Univ, State Key Lab Nat Med, Nanjing 210009, Jiangsu, Peoples R China
[3] China Pharmaceut Univ, Res Ctr Biostat & Computat Pharm, Nanjing 210009, Jiangsu, Peoples R China
关键词
ACID; STRESS; INJURY; ALPHA; CELLS;
D O I
10.1038/srep04715
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];
摘要
To investigate a possible methodology of exploiting herbal medicine and design polytherapy for the treatment of non-alcoholic fatty liver disease (NAFLD), we have made use of Cichorium glandulosum Boiss et Huet (CG), a traditional Chinese herbal medicine that has been proven to be effective in treating hepatic diseases. Here, we report that the extract of CG effectively reduced lipid accumulation under conditions of lipid overloading in vivo and in vitro (in a rat high-fat diet model and a hepG2 cell model of free fatty acid treatment). CG extract also protected hepatocytes from injury and inflammation to aid its lipid-lowering properties (in a rat high-fat diet model and a L02 cell model of acetaminophen treatment). Serum chemistry analysis accompanied by in vitro drug screening confirmed that CG-4, CG-10 and CG-14 are the lipo-effective components of CG. Western blotting analysis revealed that these components can regulate key lipid targets at the molecular level, including CD36, FATP5 and PPAR-a, thus the lipid oxidation and lipid absorption pathways. Finally, we adopted the experimental design and statistical method to calculate the best combination proportion (CG-4: CG-10: CG-14=2.065: 1.782: 2.153) to optimize its therapeutic effect.
引用
收藏
页数:10
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