Sonication of chitin-glucan, preparation of water-soluble fractions and characterization by HPLC

被引：27

作者：

Mislovicová, D ^{[1
]}

Masárová, J

Bendzálová, K

Soltés, L

Machová, E

机构：

[1] Slovak Acad Sci, Inst Chem, SK-84238 Bratislava, Slovakia

[2] Comenius Univ, Fac Nat Sci, SK-84215 Bratislava, Slovakia

[3] Slovak Acad Sci, Inst Expt Pharmacol, SK-84216 Bratislava, Slovakia

来源：

ULTRASONICS SONOCHEMISTRY | 2000年 / 7卷 / 02期

关键词：

fractionation; HPLC; sonication; water-soluble chitin-glucan;

D O I：

10.1016/S1350-4177(99)00030-9

中图分类号：

O42 [声学];

学科分类号：

070206 ; 082403 ;

摘要：

A water-insoluble chitin-glucan complex, isolated from the mycelium of Aspergillus niger, was swollen in various aqueous media and treated subsequently by high-energy sonication. The concentration of the resulting water-soluble polysaccharide fractions was dependent on the swelling medium, the amount of the chitin-glucan complex in the suspension, and on the time of sonication. The yields of water-soluble chitin-glucan were within the range 13.6 to 24.4% relative to the mass of the original chitin-glucan. The nitrogen content obtained for the samples of water-soluble chitin-glucan indicated a higher content of chitin (3.45% of nitrogen in high-molecular fraction) than in the original water-insoluble chitin-glucan sample (1.8%). The distribution of the molecular weights of the water-soluble fractions prepared was determined by high-performance liquid chromatography. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：63 / 68

页数：6

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