Isolation and characterization of a virus that infects Emiliania huxleyi (Haptophyta)

被引：78

作者：

Castberg, T

Thyrhaug, R

Larsen, A

Sandaa, RA

Heldal, M

Van Etten, JL

Bratbak, G

机构：

[1] Univ Bergen, Dept Microbiol, N-5020 Bergen, Norway

[2] Univ Nebraska, Nebraska Ctr Virol, Lincoln, NE 68583 USA

[3] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA

来源：

JOURNAL OF PHYCOLOGY | 2002年 / 38卷 / 04期

关键词：

algal bloom termination; algal virus; Emiliania huxleyi; Haptophyta; large dsDNA viruses; Phycodnaviridae; virus ecology;

D O I：

10.1046/j.1529-8817.2002.02015.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The isolation and characterization of a virus (designated EhV) that infects the marine coccolithophorid Emiliania huxleyi (Lohmann) Hay & Mohler are described. Three independent clones of EhV were isolated from Norwegian coastal waters in years 1999 and 2000. EhV is a double-stranded DNA-containing virus with a genome size of similar to415 kilo-base pairs. The viral particle is an icosahedron with a diameter of 160-180 nm. The virus particle contains at least nine proteins ranging from 10 to 140 kDa; the major capsid protein weighs similar to54 kDa. EhV has a latent period of 12-14 h and a burst size of 400-1000 (mean, 620) viral particles per cell. A phylogenetic tree based on DNA polymerase amino acid sequences indicates EhV should be assigned to the Phycodnaviridae virus family and that the virus is most closely related to viruses that infect Micromonas pusilla and certain Chlorella species.

引用

页码：767 / 774

页数：8

共 59 条

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