Geminin is essential to prevent endoreduplication and to form pluripotent cells during mammalian development

被引：103

作者：

Gonzalez, Michael A. ^{[1
]}

Tachibana, Kiku-e K.

Adams, David J.

van der Weyden, Louise

Hemberger, Myriam

Coleman, Nicholas

Bradley, Allan

Laskey, Ronald A.

机构：

[1] MRC, Canc Cell Unit, Hutchison MRC Res Ctr, Cambridge CB2 2XZ, England

[2] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England

[3] Babraham Inst, Cambridge CB2 4AT, England

来源：

GENES & DEVELOPMENT | 2006年 / 20卷 / 14期

基金：

英国医学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

geminin; endoreduplication; trophoblast; Oct4; Cdx2; pluripotency;

D O I：

10.1101/gad.379706

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In multicellular eukaryotes, geminin prevents overreplication of DNA in proliferating cells. Here, we show that genetic ablation of geminin in the mouse prevents formation of inner cell mass (ICM) and causes premature endoreduplication at eight cells, rather than 32 cells. All cells in geminin-deficient embryos commit to the trophoblast cell lineage and consist of trophoblast giant cells (TGCs) only. Geminin is also down-regulated in TGCs of wild-type blastocysts during S and gap-like phases by proteasome-mediated degradation, suggesting that loss of geminin is part of the mechanism regulating endoreduplication.

引用

页码：1880 / 1884

页数：5

共 28 条

[1] MONOCLONAL-ANTIBODIES AGAINST TROPHECTODERM-SPECIFIC MARKERS DURING MOUSE BLASTOCYST FORMATION [J].

BRULET, P ;

BABINET, C ;

KEMLER, R ;

JACOB, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (07) :4113-4117

[2] Cdx2 is essential for axial elongation in mouse development [J].

Chawengsaksophak, K ;

de Graaff, W ;

Rossant, J ;

Deschamps, J ;

Beck, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (20) :7641-7645

[3] RETRACTED: Cdx2 gene expression and trophectoderm lineage specification in mouse embryos (Retracted article. See vol 317, pg 450, 2007) [J].

Deb, K ;

Sivaguru, M ;

Yong, HY ;

Roberts, RM .

SCIENCE, 2006, 311 (5763) :992-996

[4] Direct interaction of geminin and Six3 in eye development [J].

Del Bene, F ;

Tessmar-Raible, K ;

Wittbrodt, J .

NATURE, 2004, 427 (6976) :745-749

[5] Cyclin E ablation in the mouse [J].

Geng, Y ;

Yu, QY ;

Sicinska, E ;

Das, M ;

Schneider, JE ;

Bhattacharya, S ;

Rideout, WM ;

Bronson, RT ;

Gardner, H ;

Sicinski, P .

CELL, 2003, 114 (04) :431-443

[6] Geminin predicts adverse clinical outcome in breast cancer by reflecting cell-cycle progression [J].

Gonzalez, MA ;

Tachibana, KK ;

Chin, SF ;

Callagy, G ;

Madine, MA ;

Vowler, SL ;

Pinder, SE ;

Laskey, RA ;

Coleman, N .

JOURNAL OF PATHOLOGY, 2004, 204 (02) :121-130

[7] Periodic expression of the cyclin-dependent kinase inhibitor p57Kip2 in trophoblast giant cells defines a G2-like gap phase of the endocycle [J].

Hattori, N ;

Davies, TC ;

Anson-Cartwright, L ;

Cross, JC .

MOLECULAR BIOLOGY OF THE CELL, 2000, 11 (03) :1037-1045

[8] The cell-cycle regulator geminin inhibits Hox function through direct and polycomb-mediated interactions [J].

Luo, LF ;

Yang, XP ;

Takihara, Y ;

Knoetgen, H ;

Kessel, M .

NATURE, 2004, 427 (6976) :749-753

[9] Geminin, an inhibitor of DNA replication, is degraded during mitosis [J].

McGarry, TJ ;

Kirschner, MW .

CELL, 1998, 93 (06) :1043-1053

[10] Loss of Geminin induces rereplication in the presence of functional p53 [J].

Melixetian, M ;

Ballabeni, A ;

Masiero, L ;

Gasparini, P ;

Zamponi, R ;

Bartek, J ;

Lukas, J ;

Helin, K .

JOURNAL OF CELL BIOLOGY, 2004, 165 (04) :473-482

← 1 2 3 →